丹参酮ⅡA磺酸钠对百草枯中毒大鼠肺组织5-脂氧化酶表达的影响  

Effects of sodium ⅡA sulfonate tanshinone on expression of 5 - lipoxygenase expression inparaquat poisoning rat lung tissue

作　　者：陶伍元 邓柳霞[1] 张文武[1] 

机构地区：[1]深圳市宝安区人民医院急诊科,广东深圳518101

出　　处：《中国急救医学》2017年第7期659-663,F0003,共6页Chinese Journal of Critical Care Medicine

摘　　要：目的观察丹参酮ⅡA磺酸钠（sodiumtanshinone11Asulfonate，STS）对百草枯中毒大鼠血清肿瘤坏死因子（tumornecrosisfactor-a，TNF-a）、白细胞介素-6（interleukin-6，IL-6）、白三烯B4（1eukotrieneB4，LTB4）浓度、肺组织5-脂氧化酶（5-lipoxygenase，5-LOX）蛋白及mRNA表达的影响。方法75只Sprague—Dawley（SD）雄性大鼠随机分成对照组、百草枯组、STS组，每组25只。对照组腹腔注射生理盐水2mL，百草枯组给予生理盐水稀释百草枯50mg／kg至2mL灌胃染毒，STS组在百草枯染毒后给予STS25me,／kg腹腔注射，连续注射3d。各组分别于制模后72h处死大鼠，心脏取血；腹主动脉取血行血气PO，检测；留取肺组织标本，采用HE染色，光镜下观察肺组织病理变化；酶联免疫法检测血清TNF-a、IL-6及LTB4浓度；Westernblot检测5-LOX蛋白表达量；实时荧光定量PCR法检测5-LOXmRNA表达量。结果①HE染色对照组大鼠肺组织结构清晰，肺泡无水肿；百草枯组大鼠肺内大量的炎性细胞浸润，肺间隔水肿断裂；STS组大鼠肺间质及肺泡充血水肿明显减轻，少量炎性细胞浸润。②百草枯组（52．76±3．85）mmHg及STS组（69．12±4．52）mmHg大鼠动脉血气PO，均显著低于对照组（86．45±5．23）mmHg（P〈0．01）；STS组动脉血气P02显著高于百草枯组（P〈0．01）。③百草枯组及STS组大鼠血清TNF-a、IL-6、LTB4浓度显著高于对照组（P〈0．01）；STS组大鼠血清TNF-a、IL-6、LTB4浓度显著低于百草枯组（P〈0．01）。④百草枯组（0．978±0．045）大鼠肺组织5-LOX蛋白表达量均显著高于对照组（0．780±0．022）及STS组（0．712±0．065）（P〈0．01）；而STS组大鼠肺组织5-LOX蛋白表达量低于对照组（P〈0．05）。⑤百草枯组（3．241±0．330）及STS组（2．045±0．234）大鼠肺组织5-LOXmRNA表达量均显著高于对照组（1．332±0．118）（P〈0．01）；STS组大鼠肺组织5-LOObjective To investigate the effects of sodium tanshinone Ⅱ A sulfonate （STS） on serum level about tumor necrosis factor - a （ TNF - a ）, interleukin - 6 （ IL - 6 ） and leukotriene 114 （LTB4） and 5 - lipoxygenase protein and mRNA expression in paraquat poisoning rats lung. Methods Sprague - Dawley （SD） male rats were randomly divided into control group, paraquat group, STS group. Each group was made up of 25 rats. Rats in control group were injected by 2 mL intraperitoneal injection of saline. Rats in paraquat group were given saline diluting paraquat 50 mg/kg to 2 mL lavage infected. Rats in STS group were given 25 mg/kg STS by intraperitoneal injection after paraquatpoisoning, and were injected continuously for three days. After the model was completed 72 h groups rats were executed respectively. The heart was taken, and the abdominal aorta blood was collected for PO2 inspection. The lung tissue specimens were stained by turbulence eosin （HE） staining, and then were observed the lung tissue pathology. The serum concentration of TNF - a, IL - 6 and LTB4 was detected by enzyme -linked immunoassay. 5 -LOX protein expression was detected by Western blotting testing. 5 -LOX mRNA expression was detected by real -time fluorescent quantitative PCR. Results （1）It can be seen that lung tissue structure is clear, pulmonary interstitial and alveolar was normal in the control group. In paraquat group, a large number of inflammatory cells infiltrated in the rat lungs, and pulmonary became edema and pulmonary interstitial fractured. In STS group, rat pulmonary interstitial and alveolar congestion edema relieved obviously, a small amount of inflammatory cell infiltrated. （2）Rat arterial blood gas PO2 was significantly lower in paraquat and STS groups than that in control group （ P 〈 0.01 ）. In STS group, arterial blood gas PO2 was significantly higher than that in paraquat group （ P 〈 0. O1 ）. （3）In paraquat and STS group, serum concentration of TNF - a, IL - 6 and LTB4 was sig

关 键 词：丹参酮ⅡA磺酸钠(STS) 百草枯中毒 5-脂氧化酶(5-LOX) 

分 类 号：R331.31[医药卫生—人体生理学]