4种丹参酮类化合物对U266细胞增殖的抑制作用及机制  

Inhibitory effects and mechanism of four kinds of tanshinone compounds on the proliferation of multiple myeloma cells

作　　者：黄巧容[1] 向洪[2] 李雪[3] 王银[1] 孟文彤[3] 毛声俊[1] 李慧[2] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川省医学科学院.四川省人民医院血液科,四川成都610072 [3]四川大学华西医院干细胞生物学研究室,四川成都610041

出　　处：《华西药学杂志》2017年第4期391-394,共4页West China Journal of Pharmaceutical Sciences

基　　金：四川省卫生厅项目(编号:120140)

摘　　要：目的研究丹参酮ⅡA(TAⅡA)、丹参酮Ⅰ(TAⅠ)、隐丹参酮(CTA)及二氢丹参酮Ⅰ(DIAⅠ)对U266细胞的增殖抑制作用及其可能的机制。方法采用CCK法检测4种丹参酮类化合物对U266细胞的增殖抑制率,用Annexin-V-FITC/PI检测细胞的凋亡,用PI法检测细胞周期;收集多发性骨髓瘤患者的骨髓标本,分离培养间充质干细胞(MSCs),用流式细胞仪检测4种丹参酮类化合物对MSCs分泌白介素6(IL-6)的影响。结果 4种丹参酮类化合物对U266细胞的增殖均有抑制作用;DIAⅠ较其余3种化合物的作用更强,引起细胞早期凋亡较多,细胞周期被阻滞在S期;DIAⅠ能明显抑制IL-6的分泌,其余3种化合物对IL-6的分泌作用无统计学意义。结论 DIAⅠ能明显抑制多发性骨髓细胞株U266的增殖,其抑制作用可能是通过抑制MSCs分泌IL-6而实现的。OBJECTIVE To investigate the inhibitory effects of 4 kinds of tanshinones compounds （ tanshinone Ⅰ , tanshinone ]ⅡA, cryptotanshinone and dihydrotanshinon Ⅰ ） on proliferation of multiple myelom（MM） cell line U266 and to study its possible mecha- nism. METHODS The inhibitory rates of U266 proliferation was tested by CCK - 8. The effects of tanshinones in U266 apoptosis and cell cycle change were determined by Annexin - V - FITC/PI double staining and PI staining, respectively. The flow cytometry was applied to evaluate the effects of tanshinones on the expression of IL - 6 in mesenehymal stem cells （ MSCs ）. RESULTS All tanshinones inhibited the proliferation of U266 and dihydrotanshinon I had the strongest inhibition effect. Dihydrotanshinon I also caused early apoptosis of more tumor ceils. Cell cycle analysis showed that dihydrotanshinon I blocked the cell cycle of U266 by keep- ing the cells in S phase. Dihydrotanshinon I significantly inhibited the IL - 6 secretion while tanshinone Ⅰ , tanshinone Ⅱ A, cryptotan- shinone had no obvious inhibition effects on the expressions of IL - 6. CONCLUSION Dihydrotanshinon Ⅰ has the strongest prolifer- ation inhibition effect on U266 and it inhibits the proliferation of myeloma ceils by inhibiting the secretion of IL - 6 of MSCs.

关 键 词：丹参酮 增殖抑制 细胞凋亡 细胞周期 多发性骨髓瘤 U266 间充质干细胞 白介素 二氢丹参酮Ⅰ 

分 类 号：R96[医药卫生—药理学]