一测多评法测定黄连须中的5种生物碱  

Determination of five kinds of alkaloids in Coptidis Rootlet by Quantitative analysis of multi-components with single marker

作　　者：王欣[1,2] 覃瑶[3,2] 孙建彬[4] 罗维早[2] 阳勇[2] 卿大双 李隆云[2] 

机构地区：[1]成都中医药大学药学院,四川成都610072 [2]重庆市中药研究院,重庆400065 [3]太极集团有限公司,重庆401147 [4]第三军医大学大坪医院野战外科研究所药剂科,重庆400042

出　　处：《华西药学杂志》2017年第4期417-420,共4页West China Journal of Pharmaceutical Sciences

基　　金：国家科技惠民计划项目(2013GS500102);《中国药典》2015版黄连须质量标准制定项目(2014-08);重庆市卫生局项目(2012-2-49);重庆市科委项目(2015-06)

摘　　要：目的采用一测多评法同时测定黄连须中5种生物碱(盐酸小檗碱、盐酸药根碱、盐酸表小檗碱、盐酸黄连碱和盐酸巴马汀)的含量。方法以盐酸小檗碱为内参物,采用HPLC法测定其与盐酸药根碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀的相对校正因子,利用该相对校正因子计算其他4种生物碱的含量;同时利用外标法测定5种生物碱的含量,比较两种测定方法的差异,验证一测多评法的可行性和准确性。结果在线性范围内,盐酸小檗碱与盐酸药根碱、盐酸表小檗碱、盐酸黄连碱、盐酸巴马汀的相对校正因子分别为1.128、1.008、1.070、1.025,在不同实验条件下,相对校正因子的重复性良好,5种生物碱成分含量的计算值与实测值间无显著性差异。结论一测多评法同时测定黄连须中5种生物碱的含量是可行的、准确的。OBJECTIVE To establish a Quantitative analysis of multi - components with single marker （QAMS） for the simultaneous determination of five kinds of alkaloids （ berberine, jatrorrhizine, epiberberine, coptisine and palmatine） in Coptidis Rootlet. METH- ODS An HPLC method was established to determine the five alkaloids. Berberine was used as the internal reference, the relative correction factors （RCFs） of the other four alkaloids were set and determined by HPLC. The contents of the other four alkaloids were calculated using their RCFs ,which were compared with those determined by External standard method. The feasibility and accuracy of QAMS method were verified. RESULTS Within a certain range, the RCFs of jatrorrhizine, epiberberine, coptisine and palmatine to berberine were 1. 128,1. 008,1. 070 and 1. 025 ,respectively,with the good repeatability in different experimental conditions. There was no significant differences between the QAMS method and External standard method. CONCLUSION The QAMS method is feasible and accurate for the simultaneous determination of five kinds of alkaloids in Coptidis Rootlet.

关 键 词：黄连须 一测多评 质量控制 含量测定 相对校正因子 高效液相色谱 生物碱 外标法 

分 类 号：R917[医药卫生—药物分析学]