猪脾转移因子与鸡毒支原体F弱毒疫苗株联合接种对SPF鸡淋巴细胞比率影响的研究  

Study on the effect of combined inoculation of pig spleen transfer factor and Mycoplasma gallisepticum F vaccine on SPF chicken lymphocyte ratio

作　　者：黄招玲[1] 胡美华[1] 林君瑾 廖惠珍 陈月香 刘道泉 邱艳红[3] 林伯全[1] 徐磊[1] 

机构地区：[1]福建农业职业技术学院动物科学学院,福州350119 [2]长乐市农业局农产品质量安全检测站,福建长乐350200 [3]福州市动物疫病预防控制中心,福州350002

出　　处：《福建畜牧兽医》2017年第4期8-10,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

基　　金：福建省高校杰出青年科研人才培育计划(闽教科[2015]54号);福建省中青年教师教育科研项目计划(JA15760);福建省科技计划项目(2016S0002);福建省科技计划项目(2017N0009);福州市科技计划项目(2012-N-94);福州市科技计划项目(2015-G-71);福州市科技计划项目(2016-G-64);福建农业职业技术学院科研项目(2016JS005);福清市科技计划项目(融科[2011]18号)资助

摘　　要：为了解猪脾转移因子(TF)与鸡毒支原体(MG)F弱毒疫苗株联合接种对SPF鸡淋巴细胞比率的影响,以活菌数达109ccu/m L的MG F株0.033 m L/羽与TF 0.02 m L/羽分侧点眼SPF鸡,设为A组;B组以MG F株0.033 m L/羽与生理盐水0.02m L/羽分侧点眼;C组以生理盐水0.033 m L/羽与TF 0.02 m L/羽分侧点眼;D组以生理盐水0.033 m L/羽和0.02 m L/羽对应分侧点眼,作为对照组。点眼后第7 d采血测定淋巴细胞比率。结果显示,A组(63.9%)、B组(53.8%)和C组(54.6%)的淋巴细胞比率均值分别比D组(41.5%)提高了22.4%、12.3%和13.1%。结果表明,TF与MG F株单独使用均能提高SPF鸡的淋巴细胞比率,且TF与MG F株联合使用后淋巴细胞比率提高值高于单独使用。To evaluate the effects of combined inoculation of pig spleen transfer factor （TF） and Mycoplasma gallisepticum （MG） F vaccine on SPF chicken lymphocyte ratio. This research adopted the living bacteria number reached 109 ccu/mL MG F. And the establishment of A group （0.033 mL/plume MG F strain and 0.02 mL/plume TF, eye dropping SPF chicken dividedly）, B group （0.033 mid plume MG F strain and 0.02 mL/plume saline）, C group （0.033 mL/plume saline and 0.02 mL/plume TF） and D group （0.033 mL/ plume saline and 0.02 mL/plume saline）, respectively. On 7 day post inoculation（dpi） blood lymphocyte ratio was detected. The results showed that compared with group D （41.5%）, the mean lymphocyte ratio of group A （63.9%）, group B （53.8%） and group C （54.6%） were increased by 22.4%, 12.3% and 13.1% respectively. The results showed that both TF and MG F strains could increase the lymphocyte ratio of SPF chickens individually, and the ratio of lymphocyte rate of combined inoculation TF and MG F strains was higher than that of single use.

关 键 词：鸡毒支原体 F弱毒疫苗 猪脾转移因子 联合接种 淋巴细胞比率 

分 类 号：S858.31[农业科学—临床兽医学]