钒酸盐对Ⅰ型糖尿病大鼠骨矿盐代谢及骨显微结构的影响  

The effect of vanadium on bone mineral metabolism and bone microstructure in rats with type Ⅰ diabetes

作　　者：裴祎[1] 张晓晶[1] 郑珂[1] 王巍[1] 穆帅[1] 薛峰[1] 滕松龄[1] 付勤[1] 

机构地区：[1]中国医科大学肿瘤医院/辽宁省肿瘤医院骨与软组织肿瘤科,辽宁沈阳110042

出　　处：《中国骨质疏松杂志》2017年第7期884-889,共6页Chinese Journal of Osteoporosis

摘　　要：目的评价钒酸盐对Ⅰ型糖尿病大鼠骨代谢及骨显微结构的影响。方法 45只雄性Wistar大鼠被分为3组:正常饲养组(n=15)、高血糖组(n=15)、高血糖钒酸盐治疗组(n=15)。对高血糖组和高血糖钒酸盐治疗组大鼠进行Ⅰ型糖尿病造模,正常饲养组大鼠尾静脉注射生理盐水作为对照。以8 w龄Wistar雄性大鼠按45 mg/kg体重注射佐链尿菌素,制作糖尿病大鼠模型。造模成功后,给予高血糖钒酸盐治疗组糖尿病大鼠应用钒酸盐治疗,每只大鼠按0.18 mg/(kg·d)钒计量喂养,每只大鼠通过灌胃治疗,治疗共持续12 w。正常饲养组和高血糖组则以生理盐水灌胃进行对照。连续喂养12 w,期间每周分别对3组大鼠进行体重、血糖监测。喂养结束以后,分别取3组大鼠胫骨、股骨进行Micro-CT检测、免疫组化和组织形态学检测、生物力学检测、骨矿含量等相关指标检测。结果经过12 w治疗,结果显示钒酸盐能够明显改善大鼠的高血糖状态,提高体重指标,而且治疗组平均骨小梁厚度、最大负荷载力、骨盐含量指标均明显高于高血糖组(P<0.05)。但钙盐的含量并没有显著提高(P>0.05)。结论钒酸盐能够降低高血糖大鼠的血糖值,改善糖尿病大鼠的骨代谢、骨小梁显微结构及骨组织的力学性能,可为糖尿病性骨质疏松症提供新的治疗方法。Objective The purpose of this study was to evaluate the effect of vanadium on bone microstructure and formation in streptozotocin（STZ）-induced diabetic rats.Methods Forty-five male Wistar rats were divided into three groups：normal control group（n=15）,high blood glucose group,and vanadium treatment group.Rats in high glucose and vanadium treatment groups received 45 mg/Kg of STZ to establish the diabetic model.Rats in control group received normal saline.After modelling,rats in the treatment group received 0.18 mg/kg vanadium intragastrically for 12 weeks.Body weight was examined every week.At sacrifice,the tibia and femur were subjected to micro computed tomography（micro-CT）,immunohistology,histomorphometry,mechanical testing,and bone mineral content examination.Results After the 12-week treatment,theresult showed that vanadium relieved high blood glucose status and increased body weight in diabetic rats.The average trabecular thickness,maximal loading,and bone mineral content in the vanadium-treated rats were higher than those in control rats.However,calcium level did not increase.Conclusion Vanadium decreases the blood glucose level in high-glucose rats and improves bone metabolism,trabecular bone microstructure,and bone mechanics.It provides a newmethod for the treatment of diabetic osteoporosis.

关 键 词：钒酸盐 Ⅰ型糖尿病 糖尿病性骨质疏松症 骨生物力学 骨形成 动物实验 

分 类 号：R687.3[医药卫生—骨科学]