橄榄苦苷对破骨细胞增殖影响的实验研究  

Effect of oleuropein on the proliferation of osteoclasts

作　　者：高梦颖[1] 黄惠娟[2] 谢树红[1] 

机构地区：[1]南京军区福州总医院,福建福州350000 [2]厦门大学附属东方医院,福建福州350025

出　　处：《中国骨质疏松杂志》2017年第7期896-899,共4页Chinese Journal of Osteoporosis

摘　　要：目的探讨橄榄苦苷不同药物浓度及不同作用时间对破骨细胞增殖的影响。方法破骨细胞的制备,采用sRANKL与M-CSF诱导小鼠单核细胞RAW264.7细胞获得破骨细胞。设置实验组:4组分别加入400μg/mL、200μg/mL、100μg/mL、50μg/mL橄榄苦苷,另设空白对照组。运用抗酒石酸酸性磷酸酶染液试剂盒进行破骨细胞TRAP染色鉴定,并采用Cell Counting Kit法(CCK法)检测应用不同浓度橄榄苦苷及不同作用时间抑制破骨细胞增殖的情况。结果与空白对照组比较,400μg/mL、200μg/mL、100μg/mL橄榄苦苷对破骨细胞的增殖均具有明显抑制作用。当药物作用时间为24 h及72 h时,400μg/mL橄榄苦苷对破骨细胞增殖的抑制作用最强,当药物作用时间为48 h时,200μg/mL橄榄苦苷对破骨细胞增殖的抑制作用最强,差异有统计学意义(P<0.05);50μg/mL橄榄苦苷对破骨细胞的增殖无明显抑制作用,差异不具有统计学意义(P>0.05)。结论橄榄苦苷可能通过破坏破骨细胞细胞膜的完整性抑制破骨细胞的增殖。Objective To explore the effect of different solubility and different time of oleuropein on the proliferation of osteoclasts.Methods RAW264.7 cells were induced by sRANKL and M-CSF to osteoclasts.Oleuropein of 400,200,100,and50 μg/mL was administered to the experimental groups,respectively.A blank control group was set.Osteoclasts were identified with tratrate-resistant acid phosphate staining.The proliferation of osteoclasts inhibited by different solubility and different time of oleuropein was tested with Cell Counting Kit（CCK） method.Results Compared to the control group,the proliferation of osteoclasts was significantly inhibited by oleuropein at concentrations of 400,200,and 100 μg/mL.The difference was statistically significant（P〈0.05）.At 24 h and 72 h of the treatment,the inhibiting effect of proliferation was the most obvious with 400 μg/mL of oleuropein.At 48 h of the treatment,the inhibiting effect of proliferation was the most obvious with 200 μg/mL of oleuropein.he difference was statistically significant（P〈0.05）.The proliferation of osteoclasts was not inhibited by oleuropein at concentration of 50 μg/mL.The difference was not statistically significant（P 0.05）.Conclusion Oleuropein can inhibit the proliferation of osteoclasts by destroying the integrity of cell membrane.

关 键 词：橄榄苦苷 破骨细胞 细胞增殖 

分 类 号：R915[医药卫生—微生物与生化药学]