丙泊酚对老龄大鼠认知功能及血清S100β蛋白神经特异性烯醇化酶的影响  

Effects of propofol on cognitive function,serum S100β protein and neuron-specific enolase in aged rats

作　　者：田首元[1] 王鑫[1] 朱健[1] 孟玉洁[1] 井子夜[1] 杨静[1] 高艳[1] 焦丽媛[1] 

机构地区：[1]山西医科大学第一医院麻醉科,太原030001

出　　处：《中国药物与临床》2017年第7期949-953,共5页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关计划项目(20100103)

摘　　要：目的探讨丙泊酚对老年大鼠认知功能及S100β蛋白、神经特异性烯醇化酶的影响。方法选用50只健康雄性Wistar大鼠,20月龄,体质量560~610 g,采用随机数字表法分为对照组(C组)25只和丙泊酚组(P组)25只。丙泊酚组腹腔注射60 mg/kg(6 ml/kg)丙泊酚,然后酌情追加,维持麻醉3 h。对照组腹腔注射等量0.9%氯化钠注射液(6 ml/kg)。于停药后1天开始行跳台实验(第1和7天)和Morris水迷宫实验(连续7 d)测试认知功能。于麻醉结束后的第1天和第7天测定血浆样本中S100β和神经特异性烯醇化酶(NSE)的浓度。结果与C组比较,P组丙泊酚麻醉结束后1 d时老龄大鼠跳台实验学习和记忆阶段潜伏期、受电击总时间均明显延长,错误次数增多(P<0.01)。麻醉结束后7 d时,2组间各指标差异无统计学意义(P>0.05)。丙泊酚麻醉结束后第1天老龄大鼠Morris水迷宫实验逃避潜伏期和总游泳距离明显延长(P<0.01),而2组大鼠在第7天水迷宫空间探索实验中原平台象限探索时间、探索距离、穿越平台次数间差异无统计学意义(P>0.05)。2组大鼠在丙泊酚麻醉后第1天和第7天血清S100β和NSE浓度差异无统计学意义(P>0.05)。结论丙泊酚麻醉后第1天老龄大鼠的被动回避能力和空间学习记忆能力可能会下降,但不会造成长时间(第7天)的影响;血清S-100β蛋白和NSE浓度在预测丙泊酚麻醉是否影响认知功能的价值不大。Objective To investigate the effects of propofol on the cognitive function, serum S10013 protein and neuron-specific enolase （NSE） in aged rats. Methods Fifty healthy male Wistar rats aged 20 months and weighed 560-610 g were randomly divided into 2 groups （n=25 each）： the control group （group C） and propofol group （group P）. The group P was injected intraperitoneally with 60 mg/kg （6 ml/kg） propofol, and then added as appropriate, to main tain anesthesia for 3 h. The control group was injected intraperitoneally with the same volume of normal saline （6 ml/kg）. The cognitive function was tested by step-down test （1 and 7 d） and Morris water maze experiment （continued 7 d） at 1 d after the drug withdrawal. The concentrations of S10013 and NSE in the plasma samples were measured at 1 and 7d after the anesthesia. Results Compared with group C, the latency and total time of electric shock of the aged rats were significantly prolonged and the number of errors increased （P〈0.01） in both learning phase and memory phase of the step-down test at 1 d after propofol anesthesia in group P. There was no statistically significant difference in the indexes between the two groups at 7 d after the anesthesia （P〉0.05）. The escape latency and total swimming distance of the Morris water maze experiment in the aged rats at 1 d after the anesthesia were significantly prolonged （P〈0.01）. There were no statistically significant differences in the time and distance of staying at the original platform quadrant, and the number of crossing the platform between the two groups at 7 d after the water maze space exploration experiment （P〉0.05）. There were no statistically significant differences in the serum S10013 and NSE concentrations between the two groups at 1 and 7 d after the propofol anesthesia （P〉0.05）. Conclusion The ability of passive avoidance and spatial learning and memory in aged rats at 1 d after propofol anesthesia may decrease, which will not cause long-term effect

关 键 词：二异丙酚 认知 大鼠 磷酸丙酮酸水合酶 S100Β蛋白 

分 类 号：R965[医药卫生—药理学]