细胞因子受体CXCR3过表达对胃癌患者预后的研究  

Over expression of the chemokine receptor CXCR3 and its correlation with favorable prognosis in gastric cancer

作　　者：王昌敏[1] 赵进[1] 黄睿[1] 

机构地区：[1]新疆维吾尔自治区人民医院临床检验中心,乌鲁木齐830001

出　　处：《检验医学与临床》2017年第14期2064-2066,2070,共4页Laboratory Medicine and Clinic

摘　　要：目的探讨细胞因子受体CXCR3过表达对胃癌患者预后的临床意义。方法采用免疫组织化学方法检测96例胃癌患者肿瘤组织及临近非肿瘤组织的CXCR3及其变体形式表达情况,通过qRT-PCR方法检测40例新鲜胃癌标本及临近非肿瘤组织CXCR3mRNA水平,采用Western blotting检测10例胃癌组织及临近非肿瘤组织CXCR3蛋白表达情况。结果与临近非肿瘤组织比较,肿瘤组织CXCR3表达均上升,且CXCR3过表达与肿瘤侵袭(P=0.030)和迁移率(P=0.019)呈负相关,与患者总生存率(P=0.001)改善呈正相关。多变量统计分析结果显示CXCR3是胃癌患者疾病预后的独立因素[HR=0.379(0.196~0.734);P=0.004]。CXCR3 2种变体CXCR3-A和CXCR3-B在胃癌组织表达中均显著上升(P=0.006,0.002),其中CXCR3-B mRNA水平显著高于CXCR3-A,CXCR3-B/CXCR3-A平均比例为1.80。结论 CXCR3表达检测对胃癌患者疾病预后的具有临床价值,且CXCR3-B表达上升提示患者疾病预后良好。Objective The purpose of this study is to investigate the clinical significance of the expression of cytokine receptor CXCR3 in the prognosis of gastric cancer patients.Methods In the present study,the expressions of CXCR3 and its variants were detected in 96 GC and corresponding nontumor gastric tissues by immunohistochemical staining,in 40 freshly frozen GC and nontumor gastric tissues by reverse-transcription polymerase chain reaction and quantitative real-time polymerase chain reaction,and in 10 freshly frozen GC and nontumor gastric tissues by Western blotting.Results The study revealed that an over expression of CXCR3 occurs in GC tissues as compared to the nontumor gastric.High level of CXCR3 expression was found to be inversely associated with invasion depth and metastasis（P=0.030 and P=0.019,respectively） and directly associated with improved overall survival （log-rank test,P〈0.001）.Furthermore,multivariate analysis showed that high CXCR3 expression acts an independent prognostic factor for GC patients [hazard ratio,0.379（0.196-0.734）,P=0.004].The messenger RNA expression of both the CXCR3 variants,CXCR3-A and CXCR3-B,were up-regulated in GC tissues（P=0.006 and P=0.002,respectively）,although CXCR3-B messenger RNA expression was significantly higher than CXCR3-A,with an average CXCR3-B to CXCR3-A ratio of 1.80.Conclusion Our study suggests a potential use of CXCR3 overexpression as a prognostic marker for GC and involvement of the up-regulation of CXCR3-B in favorable prognosis of GC patients.

关 键 词：CXCR3 胃癌 预后 转移 

分 类 号：R512.62[医药卫生—内科学]