MERS-CoV棘突蛋白主要特性及B/T细胞抗原表位预测分析  

Characteristics and prediction of B-and T-cell epitopes of the Spike protein from MERS-CoV

作　　者：于娟[1] 李红[1] 卢囡囡 饶华祥[1] 雷有菊[1] 姜双应 赵生仓 

机构地区：[1]青海省疾病预防控制中心,青海西宁810007

出　　处：《中国病原生物学杂志》2017年第7期627-630,共4页Journal of Pathogen Biology

基　　金：国家"十二五"科技重大专项(No.2012zx10004-208)

摘　　要：目的应用生物信息学方法分析中东呼吸综合征冠状病毒(Middle East respiratory syndrome coronavirus,MERS-CoV)棘突蛋白(Spike,S)的主要特性,预测可能的B/T细胞抗原表位。结果 MERS-CoV的S蛋白氨基酸序列基本保守,具有多个糖基化、酰胺化及磷酸化翻译后修饰位点。综合各种单参数预测MERS-CoV S蛋白可能存在4个B细胞抗原表位、7个CTL细胞表位和5个Th细胞表位。方法以MERS-CoV S蛋白的氨基酸序列一级结构为基础分析其保守性,采用ProtParam预测S蛋白的理化特性,SOPMA预测其二级结构,MotifScan预测翻译后修饰位点,DNAStar分析其序列的亲水性指数、柔韧性指数、可及性参数以及抗原指数,推测B细胞抗原表位的可能位置,采用SYFPEITHI的T细胞表位预测工具预测CTL和Th细胞表位。结论生物信息学方法预测MERS-CoV S蛋白氨基酸保守序列,该蛋白可能含有B/T细胞抗原表位,为MERS-CoV疫苗研制及其他相关研究奠定了基础。Objective To predict the main characteristics and B- and T-cell epitopes of the Spike protein （S） of Middle East respiratory syndrome coronavirus （MERS-CoV）. Methods Analysis of the extent to which the S protein of MERS-CoV was conserved was performed based on its primary structure. Physical and chemical properties were predicted using ProtParam, and the secondary structure of the protein was predicted using SOPMA online software. Post-transla- tional sites were predicted using the software MotifScan. B-cell epitopes were analyzed using the software DNAStar, which was used to analyze hydrophilicity, flexible regions, surface-accessible regions, and the antigenic index. T-cell epitopes were analyzed using SYFPEITHI. Results The amino acid sequence of the S protein from MERS-CoV was conserved, and mutations at 23 sites were evident in more than two MERS-CoV isolates. The protein had several post- translational sites, including 23 N-glycosylation sites, 21 N-myristoylation sites, and 40 phosphorylation sites. Four po- tential B-cell epitopes, 7 potential CTL cell epitopes, and 5 Th cell epitopes were predicted for the S protein. Conclusion The characteristics of the S protein and prediction of its B- and T-cell epitopes might help to develop promising anti- MERS vaccines based on peptides.

关 键 词：MERS-CoV SPIKE蛋白 B/T细胞表位 生物信息学 

分 类 号：R373.1[医药卫生—病原生物学]