口腔菌群结构动态变化与胃癌发生发展相关性分析  

Analysis of the oral microbiota and its potential association with gastric cancer

作　　者：杨俊杰[1,2] 张敬美[1,3] 陈斌[1] 孟庆松[4] 石峰 史国生 张磊[1,5] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]齐鲁师范学院生命科学学院 [3]济宁医学院行为医学教育研究所 [4]山东省千佛山医院 [5]山东省食品药品检验研究院

出　　处：《中国病原生物学杂志》2017年第7期631-634,共4页Journal of Pathogen Biology

基　　金：山东省自然科学基金项目(No.ZR2015CL005)

摘　　要：目的探讨胃癌患者口腔菌群结构动态变化与胃癌的相关性。方法采用微生物鉴定芯片技术高通量分析和比较胃癌患者和健康对照受试者唾液中菌群结构动态变化与胃癌发生发展相关性;运用SPSS软件对基因芯片中424种微生物探针信号进行分析;采用dChip软件分析口腔菌群差异,采用MEGA软件制作系统进化树进行多样性分析。结果与对照组比较,胃癌组患者的唾液中有26种菌群显著减少,差异有统计学意义。其中包括拟杆菌门(Bacteroidetes)5种、变形菌门(Proteobacteria)8种、放线菌门(Actinobacteria)1种、厚壁菌门(Firmicutes)9种、梭杆菌门(Fusobacteria)3种。结论胃癌患者口腔菌群发生变化,有显著变化的细菌种类有望做为胃癌检测的生物标志物。Objective Recent studies on variations in oral microbiota have indicated its potential role in pathogenesis and early detection of gastric diseases. The aims of this study were to measure variations in oral microbiota and to evaluate its potential association with gastric cancer. Methods Variations in salivary microbiota were examined in 17 patients with gastric cancer and 32 healthy controls using the Human Oral Microbe Identification Microarray （HOMIM）. SPSS was used to analyze the microarray signals of 424 microbial probes. Differences in oral flora were analyzed using dChip, and a phylogenetic tree was constructed using MEGA. Results Comprehensive comparison of the salivary microbiota in patients with gastric cancer and controls revealed a significant variation in salivary microfiora. Of 424 oligonucleotide probes in the HOMIM, 26 probes targeting different species or higher taxa produced significantly decreased signals in the saliva pellets of patients with gastric cancer in comparison to those of the healthy controls. These species/clusters belong to five different bacterial phyla, namely, Bacteroidetes （5 species/clusters）, Proteobacteria （8 species/clusters）, Acti- nobacteria （1 species/cluster）, Firmicutes （9 species/clusters）, and Fusobacteria （3 species/clusters）. Results Com- prehensive comparison of the salivary microbiota in patients with gastric cancer and controls revealed a significant variation in salivary microfiora. Of 424 oligonucleotide probes in the HOMIM, 26 probes targeting different species or higher taxa produced significantly decreased signals in the saliva pellets of patients with gastric cancer in comparison to those of the healthy controls. These species/clusters belong to five different bacterial phyla, namely, Bacteroidetes （5 species/clus- ters）, Proteobacteria （8 species/clusters）, Actinobacteria （1 species/cluster）, Firmicutes （9 species/clusters）, and Fu- sobacteria （3 species/clusters）. Conclusion An association between variations i

关 键 词：胃癌 口腔菌群 多样性分析 

分 类 号：R378[医药卫生—病原生物学] R735.2[医药卫生—基础医学]