2,4-二叔丁基苯酚为内标高效液相色谱法测定丙泊酚血药浓度  

Detection of propofol plasma concentration by HPLC with 2, 4-ditert-butyl phenol as internal standard

作　　者：万海方[1] 黄焱明[1] 汪国香[1] 罗宏[1] 张相彩[2] 陶凡[1] 赵萃[3] 王振[1] 

机构地区：[1]浙江省中西医结合医院杭州市红十字会医院麻醉科,浙江杭州310003 [2]浙江省中西医结合医院临床药学研究室,浙江杭州310003 [3]浙江工业大学药学院绿色制药技术与装备教育部重点实验室长三角绿色制药协同创新中心,浙江杭州310014

出　　处：《中国现代医生》2017年第20期105-108,共4页China Modern Doctor

基　　金：浙江省医药卫生科技计划项目(2015KYA187);浙江省杭州市科学技术委员会医疗卫生及重点专科专病科研攻关项目(20130733Q13);浙江省杭州市卫生计生科技计划(2016A34)

摘　　要：目的建立以2,4-二叔丁基苯酚为内标的高效液相色谱法测定丙泊酚血药浓度方法。方法血清样品用乙腈-甲醇(75:25)混合液蛋白沉淀后取上清液进样分析,以2,4-二叔丁基苯酚为内标,采用Ultimate~XB-C_(18)色谱柱(4.6 mm×250 mm,5μm),以水(H_3PO_4调pH=1.8)-乙腈(70∶30)为流动相,流速1.5 mL/min,检测激发波长270 nm。结果该法专属性高,回收率高,日内日间RSD低。丙泊酚血药浓度在0.2~10.0 mg/L范围内线性关系良好(r^2=0.9998),最低检测浓度0.2970 mg/L。结论本方法测定丙泊酚浓度方便、快捷、准确度好、精密度高,为开展血药浓度检测、药动学研究提供方法学依据。Objective To establish a detecting method of propofol plasma concentration by HPLC with 2, 4-ditert-butyl phenol as internal standard. Methods The supernatant of serum samples were analyzed after protein precipitation by acetonitrile-methyl alcohol（75：25） mixed liquor. With 2, 4-ditert-butyl phenol as internal standard, Ultimate XB-C18 chromatographic column（4.6 mmx250 mm, 5 μm） was used. Water（H3PO4, pH=1.8）-acetonitrile（70：30） was used as mobile phase, flow velocity=l.5 mL/min, and detecting excitation wave length=270 nm. Results This method had high specificity and recovery, with low intra-day and inter-day RSD. When the plasma concentration of propofol was between 0.2-10.0 mg/L, it showed a good linear relation （r2=0.9998）, and the lower limit of detection was 0.2970 mg/L. Conclusion This method is convenient and speedy, with high accuracy and precision, providing a methodological basis for plasma concentration detection and pharmacokinetic research.

关 键 词：丙泊酚 高效液相色谱法 血药浓度 2 4-二叔丁基苯酚 

分 类 号：R614[医药卫生—麻醉学]