骨髓多能间质细胞促进同种异体移植胰岛新生血管形成的机制  

Mechanism of mesenchymal stromal cells in promoting islet graft revascularization

作　　者：李睿[1] 董红丽[2] 刘宝林[3] 

机构地区：[1]中国医科大学人民医院肝胆外科,沈阳110016 [2]中国医科大学附属盛京医院社会服务部 [3]中国医科大学附属盛京医院胆道、血管外科

出　　处：《中华肝胆外科杂志》2017年第7期474-477,共4页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的探讨骨髓多能间质细胞（MSCs）促进同种异体移植胰岛新生血管形成的相关机制。方法以非肥胖糖尿病（NOD／Lt）小鼠作为受体，诱导免疫耐受之后给予同种异体胰岛移植并联合MSCs移植，应用流式细胞仪检测淋巴细胞嵌合率，应用免疫荧光检测MSCs迁移、转化及分泌细胞因子情况。结果联合MSCs移植组小鼠淋巴细胞嵌合率明显增高，经羧基荧光素二乙酸盐琥珀酰亚胺酯（CFDA-SE）标记的MSCs可同时被抗α-actin抗体标记或抗VEGF抗体标记。结论MSCs可迁移到移植胰岛周围，并通过转化为血管平滑肌细胞，以及通过分泌VEGF来促进同种异体胰岛移植物的血管重建。MSCs还可增加NOD小鼠的供体淋巴细胞嵌合水平，进而增强同种异体胰岛移植物的免疫耐受。Objective To investigate the mechanisms of mesenchymal stromal cells （MSCs） in promoting the transplanted islets neovascularization. Methods We performed co-transplantation of allogeneic islet and MSCs into non-obese diabetic （NOD） mice and the lymphocyte chimeric rate was detected by flow cytometry. The MSCs migration, transformation and secretion of cytokines were detected by immunofluorescence. Results In mice of co-combination transplantation of islet with MSCs, donor lymphocytic chimerism in graft was increased. BM-MSCs can migrate to transplanted islet, differentiate into vascular smooth muscle cells, and also secrete vascular endothelial growth factor （VEGF）. Conclusions MSCs can migrate to transplanted islet and promote neovascularization. Also, it enhanced allograft immune tolerance of islet grafts via increasing donor lymphocytic chimerism.

关 键 词：I型糖尿病 胰岛移植 骨髓多能间质细胞 

分 类 号：R587.1[医药卫生—内分泌]