CD46、Nectin-4在麻疹病毒感染人肺泡上皮细胞中的作用  

Role of CD46 and Nectin-4 on Measles virus(MV) infecting human pulmonary alveolar epithelial cells

作　　者：蔡天晶[1] 黎志良[1] 金凌应[1] 甘云波[2] 

机构地区：[1]湖北省通城县人民医院,通城437400 [2]湖北文理学院医学院,襄阳441000

出　　处：《中国免疫学杂志》2017年第7期991-994,共4页Chinese Journal of Immunology

基　　金：湖北省卫生与计划生育委员会项目(No.WJ2015MB301)

摘　　要：目的:探讨CD46、Nectin-4在麻疹病毒感染人肺泡上皮细胞中的作用。以及CD46和Nectin-4之间的相互作用。方法:分为麻疹病毒感染前处理组和感染后2 h组,均用抗CD46抗体、抗Nectin-4抗体处理人肺泡上皮细胞,并设对照组。检测细胞内病毒滴度的变化,采用Western blot和Real-time PCR检测NP蛋白的表达水平,并运用免疫共沉淀检测CD46与Nectin-4的相互作用。结果:与对照组相比,麻疹病毒感染前用抗CD46抗体、抗Nectin-4抗体处理的人肺泡上皮细胞内麻疹病毒滴度显著降低,分别为对照组的48.03%和49.53%,同时用抗CD46抗体、抗Nectin-4抗体处理使病毒滴度下降为27.15%,差异均有统计学意义(P<0.01)。然而,在麻疹病毒感染2 h后用抗CD46抗体、抗Nectin-4抗体处理,人肺泡上皮细胞内麻疹病毒滴度和NP蛋白量未见显著减少。Western blot和Real-time PCR结果与上述结果一致;免疫共沉淀实验显示,CD46与Nectin-4存在相互作用。结论:CD46、Nectin-4在麻疹病毒感染肺泡上皮细胞中起到介导作用,CD46与Nectin-4可能存在协同作用,该作用对麻疹病毒的感染起到了增强效果。Objective： To investigate the role of CD46 and Nectin-4 on Measles virus （MV） infecting human pulmonary alveolar epithelial ceils （HPAEpiC）, and the interaction between CD46 and Nectin-4. Methods： Measles virus was divided into pre- infection group and 2 h-infection group, HPAEpiCs treatment with anti-CD46 antibody and/or anti-Nectin-4 antibody as experimental groups, and untreated HPAEpiCs as a control. The variation of viral replication level was detected. A Co-immunoprecipitation assay （ Co- IP） was used to explore whether CIM6 and Nectin-4 had interactive relationship in MV infection. Results： Compared with the control group, MV titers were reduced in HPAEpiCs of the pre-infection group treated with anti-CIM6 and anti-Nectin-4 respectively （48. 03% and 49.53% ）. Furthermore, virus titers showed a more reduction in which treated with anti-CIM6 and anti-Nectin-4 antibodies （27. 15%~,P〈0. 01 ）. Western blot and Real-time PCR showed that anti-CIM6 antibody and anti-Nectin-4 antibodies decreased the rate of MV infection. In the 2 h-infection group,however,the treatment with anti-CIM6 and anti-Nectin-4 could significantly reduce the MV titer and NP protein in HPAEpiCs. The Co-IP assay showed that there were interaction between CD46 and Nectin-4. Conclusion： CIM6 and Neetin-4 mediated MV infecting HPAEpiCs. Moreover,CD46 and Nectin-4 may play a synergetic role in MV infection,which could enhance the infection effect.

关 键 词：麻疹病毒 人肺泡上皮细胞 CD46 Nectin-4 

分 类 号：R373.1[医药卫生—病原生物学]