无刺枸骨叶片结构对自然变温的适应  

Adaptability of Leaf Morphology of Ilex cornuta var. fortune to Ambient Air Temperature Change

作　　者：李璐璐[1] 姜新强[1] 李绍翠 刘庆超[1] 刘庆华[1] 王奎玲[1] 

机构地区：[1]青岛农业大学园林与林学院,山东青岛266109

出　　处：《中国农学通报》2017年第16期71-77,共7页Chinese Agricultural Science Bulletin

基　　金：青岛市民生科技计划项目"彩叶和常绿阔叶植物的引种繁育栽培技术研究与示范"(14-2-3-36-nsh);山东省农业良种工程重大课题"林木种质资源收集保护与评价"(鲁农良字[2010]6号)

摘　　要：为阐明自然低温胁迫条件下无刺枸骨植株表现与其低温适应性,以无刺枸骨(Ilex cornuta var.fortunei)叶片为试材,通过田间形态观测、电导率测定和常规石蜡切片法,研究其植株形态、叶片细胞膜透性和叶片解剖结构在自然变温过程中的适应性变化。结果显示,随着温度的逐渐降低,无刺枸骨叶色、叶片相对电导率均受到不同程度的影响,3月叶片相对电导率最高,为44.29%;自然变温过程中无刺枸骨叶片厚度、上、下角质层厚度、上、下表皮细胞厚度、栅栏组织厚度、海绵组织厚度、叶片组织紧密度、栅海比(栅栏组织厚度/海绵组织厚度)等指标均表现出先上升后下降的趋势,而细胞疏松度先降低后增大,各参数在不同月差异显著。上述研究表明,无刺枸骨可以通过叶片结构的适应性变化提高自身对低温逆境的适应性。To illustrate the morphological and leaf anatomical structure changes of Ilex cornuta var. fortunei and their adaptability to the changes of ambient air temperature, this study investigated the morphological andhistological structure of the leaves of I. cornuta var. fortunei. The methods included field morphologicalobservation and paraffin section. The results showed that as the temperature gradually decreased, the leaf colorand relative electrolytic leakage changed significantly during the changes of ambient air temperature. Thehighest relative electrolytic leakage was 44.29% in March 2015. In addition, different leaf tissue structureindexes, including leaf thickness, upper and lower epidermis thickness, upper and lower cuticle thickness,palisade tissue thickness, spongy tissue thickness and ratio of palisade tissue to spongy tissue were examined.These different indexes increased initially and then decreased with the gradual temperature decrease.However, the spongy ratio tended to decrease, and then increase. These indicators changed significantly underdifferent months. Therefore, the results indicated that improved adaptability of cold tolerance in I. cornuta var.fortunei relied on the changes of leaf structure.

关 键 词：无刺枸骨 相对电导率 叶片结构 

分 类 号：S687.2[农业科学—观赏园艺]