辣椒Trihelix转录因子家族的生物信息学分析  

Bioinformatics Analysis of Trihelix Transcription Factors from Chilli Pepper

作　　者：张春利[1,2] 袁岐[1,2] 赵婷婷[1,2] 许向阳[1,2] 

机构地区：[1]东北农业大学园艺园林学院,哈尔滨150030 [2]东北农业大学农业部东北地区园艺作物生物学与种质创制重点实验室,哈尔滨150030

出　　处：《中国农学通报》2017年第17期32-38,共7页Chinese Agricultural Science Bulletin

基　　金：现代农业产业技术体系专项基金"国家大宗蔬菜产业技术体系岗位专家"(CARS-25-A-15);国家自然科学基金"抗叶霉病基因Cf19的克隆功能验证及防御信号传导途径的研究"(31272171);黑龙江省大宗蔬菜新品种选育项目"黑龙江省大宗蔬菜新品种选育"(GA15B103-1);农业部东北地区园艺作物生物学与种质创制重点实验室开放课题基金项目资助

摘　　要：Trihelix转录因子在调控植物生长发育以及响应逆境胁迫等多方面发挥着重要作用,属于一个小家族。通过系统地阐述辣椒Trihelix转录因子家族成员特征和进化关系,进一步为研究辣椒Trihelix转录因子的生物学功能提供理论基础。本研究在辣椒基因组范围内,通过HMMER 3.0软件鉴定Trihelix基因家族成员,应用CDD验证其蛋白的功能结构域;采用MEGA5.2软件进行系统进化树分析;利用MEME和Map Inspect工具对其蛋白序列进行基序和染色体定位分析。利用功能已知的拟南芥Trihelix蛋白家族为参考序列,系统分析鉴定了28个辣椒Trihelix家族基因,并将其分为GT-1、GT-2、GTγ、SIP1和SH4 5个亚族。基因定位表明,辣椒Trihelix家族成员不均匀的分布在12条染色体上,其中7号和11号染色体上没有辣椒Trihelix成员的分布。保守元件显示辣椒Trihelix基因家族成员部分是高度保守的,各个家族均具有特殊的结构域。辣椒Trihelix基因家族大部分成员结构是保守的,保守基序与聚类分析具有高度的一致性,有可能参与调控辣椒生长发育及逆境胁迫等多种过程。Trihelix transcription factors play a significant role in the regulation of plant growth and development, as well as the response to environmental stresses, and they belong to a small family. In order to provide a theoretical basis for further study on biology function of pepper Trihelix transcription factors, this paper systematically elaborated the member characteristics of Trihelix transcription factor family and evolutionary relationships of pepper. Based on the pepper genome database, Trihelix gene family was identified using HMMER 3.0 software and the functional domain of the protein was verified by CDD. A phylogenetic tree was established using the MEGA5.2 software. Motif and chromosomes locations of protein sequence were done with MEME and Maplnspect tools. 28 Trihelix family genes were identified systematically from pepper, then were classified into GT-1, GT-2, GTT, SIP1 and SH4 five subfamilies according to the known Arabidopsis thaliana Trihelix protein family as reference sequences. Gene mapping analysis showed that pepper Trihelixgenes were distributed unevenly on 12 chromosomes, among which there was no distribution of pepper Trihelix members on chromosomes 7 and 11. Conserved components analysis indicated that part of pepper Trihelix genes family was highly conserved, and each subfamily had a special domain. These results suggested that the majority of pepper Trihelix genes were highly conserved, and conservative motif and the cluster analysis had a consistence of high degree and might participated in the multiple regulation process of growth, development and environmental stress in pepper.

关 键 词：辣椒 Trihelix 转录因子 进化 生物信息学分析 

分 类 号：S641.3[农业科学—蔬菜学]