脐血单核细胞治疗脊髓损伤的临床观察  

The clinical observation of treatment spinal cord injury with transplantation of human umbilical cord blood mononuclear cells

作　　者：刘斌[1] 刘波[2,3] 段答[2,3] 樊梦瑶[2,3] 徐蓉[1] 滕晓华[2,3] 卢明[2,3] 

机构地区：[1]湖南师范大学医学院,湖南长沙410006 [2]湖南师范大学第二附属医院 [3]解放军第163医院神经外科,湖南长沙410003

出　　处：《湖南师范大学学报（医学版）》2012年第1期62-65,共4页Journal of Hunan Normal University(Medical Sciences)

摘　　要：目的:观察人脐血单核细胞(Mononuclear cells,MNCs)移植治疗脊髓损伤(spinal cord injury)的疗效。方法:收集正常足月剖腹产胎儿的脐带血,经肝素抗凝,用淋巴细胞分离液分离脐血的单个核细胞,采用差速贴壁法进行纯化,并经腰椎穿刺注入患者体内。结果:治疗后患者肢体自主运动、感觉及两便的控制功能明显改善,与治疗前相比差异具有统计学意义(P<0.05)。结论:脐血MNCs移植治疗对部分SCI患者恢复具有较好的治疗效果。Objective To observe the therapeutic effect of spinal cord injury by human umbilical cord blood mononuclear cells transplantation.Methods Cells obtained from full term deliveries scheduled for cesarean section were cultured,all specimens were obtained sterilely with preservative-free heparin,the mononuclear cells were isolated by lymphocyte separation medium,purified by Nash differential adhesion,and then injected into cavitas subarachnoidealis of 37 patients.Results The body autonomy movement,feeling and the size of contro function significantly improved after treatment,compared with treatment before the difference with statistically significant(P<0.05).Conclusion Mononuclear cells transplantation has therapeutic effect to some patients with spinal cord injury.

关 键 词：人脐血单核细胞 脊髓损伤 

分 类 号：R651.2[医药卫生—外科学]