桉树焦枯病菌ABC转运蛋白的鉴定与分析  

Identification and Analysis of ABC Transporter in Calonectria pseudoreteaudii

作　　者：刘宏毅[1] 陈全助[2] 叶小真[2] 陈慧洁[1] 李慧敏[1] 冯丽贞[1] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学金山学院,福建福州350002

出　　处：《林业科学研究》2017年第4期685-692,共8页Forest Research

基　　金：福建省财政厅项目(K81150002;K81139238;K8112004A)

摘　　要：[目的]利用生物信息学方法探讨ABC转运蛋白在焦枯病菌对桉树侵染过程中的解毒作用,为揭示桉树焦枯病致病机制奠定基础。[方法]本文利用BLAST、HMMER、Pfam数据库、TCDB数据库在全基因组内对桉树焦枯病菌的ABC转运蛋白进行鉴定和分类,并通过Prot Camp和IBS分别进行亚细胞定位和结构域图绘制,依据同源性对其功能进行推测分析。[结果]表明:桉树焦枯病菌共有70个ABC转运蛋白,它们分属于8个亚族。71%的转运蛋白位于细胞膜,10%位于液泡,其余部分位于线粒体、过氧化物酶体、内质网等内膜系统上。桉树焦枯病菌ABC转运蛋白包含全分子、四分之三分子、半分子及四分之一分子。根据同源性推测桉树焦枯病菌ABC转运蛋白与MDR、PDR、HMT、MPE、STE、P-FAT等几种转运蛋白具有较高相似性,同时还与核糖体合成、翻译、过氧化物酶体合成的ABC转运蛋白具有较高相似性。[结论]桉树焦枯病菌ABC转运蛋白除作为外排泵转运外源化学物质、疏水性化学分子等物质。还参与细胞代谢、翻译、核糖体的合成、mRNA的输出、β氧化,并通过为辅酶Q的辅助因子增强呼吸代谢等多项生命活动为焦枯病菌的侵染提供能量。[Objective] Using bioinformatics method to investigate the detoxification of ABC transporters in Calonectria during the process infecting Eucalyptus, and to provide references for revealing the pathogenic mechanism of Calonectria. [Method] Using BLAST, HMMER, Pfam database, TCDB database to indentify and classify the ABC transporter of Calonectria within the whole genome, and to predict subcellular localization and the domain structure scheme with ProtCamp and IBS, respectively. [Result] Calonectria has a total of 70 ABC transporters, which belong to 8 subfamilies. 71% of the transporters are located in the cell membrane, 10% in the vacuole, and the rest in the mitochondria, peroxisome, endoplasmic reticulum and other endometrial systems. Calonectria ABC transporter has 4 types of molecules, contains full-size molecules, 3/4 molecules, half molecules and 1/4 molecules. According to the homology it is estimate that Calonectria ABC transporters has high similarity with MDR, PDR, HMT, MPE, STE, P-FAT and other transporters. It also has high similarity with the ABC transporters related to the synthesis of ribosome, translation and synthesis of peroxisome. [Conclusion] The ABC transporter of Calonectria pseudoreteaudii can transport exogenous chemicals, hydrophobic chemical molecules. It is also involved in cell metabolism, translation, ribosome synthesis, the export of mRNA, beta-oxidation, and provide energy for infecting as a cofactor of coenzyme Q.

关 键 词：桉树 焦枯病菌 ABC转运蛋白 结构域 亚细胞定位 

分 类 号：S763.7[农业科学—森林保护学]