七氟醚后处理对大鼠脑缺血-再灌注时氧化应激和炎症反应的影响  

Effects of sevoflurane post-conditioning on oxidative stress and inflammatory reaction during rat cerebral ischemia-reperfusion

作　　者：赵丹[1] 袁林辉[2] 张静[2] 张平[3] 余鹏[2] 肖凡[2] 胡小玲[1] 胡衍辉[2] 

机构地区：[1]江西省肿瘤医院麻醉科,南昌市330000 [2]南昌大学第二附属医院麻醉科 [3]南昌大学第一临床医学院

出　　处：《临床麻醉学杂志》2017年第7期688-692,共5页Journal of Clinical Anesthesiology

基　　金：江西省卫计委项目(20165284)

摘　　要：目的评价七氟醚后处理对大鼠脑缺血-再灌注时氧化应激及炎症反应的影响,以探讨其脑保护机制。方法健康雄性清洁级SD大鼠36只,12~14周龄,体重220~260g,采用随机数字表法分为假手术组(Sham组)、脑缺血-再灌注组(IR组)和脑缺血-再灌注+七氟醚后处理组(SPC组),每组12只。制备大鼠脑缺血-再灌注损伤模型,缺血30min后再灌注24h。Sham组不阻塞大脑中动脉;IR组:建立脑缺血-再灌注损伤模型;SPC组于再灌注即刻给予2.6%七氟醚吸入15min。再灌注末处死各组大鼠,断头取出脑组织。采用Western blot法检测Iba-1和HO-1蛋白含量;并测定脑组织中活性氧(ROS)含量,丙二醛(MDA)、TNF-α、IL-1β浓度和超氧化物歧化酶(SOD)活性。结果 IR组和SPC组脑皮质Iba-1蛋白含量明显高于Sham组(P<0.05),SPC组Iba-1蛋白含量明显低于IR组(P<0.05)。与Sham组比较,IR组和SPC组ROS含量和MDA、TNF-α、IL-1β浓度明显升高,SOD活性和HO-1蛋白含量明显降低(P<0.05)。SPC组ROS含量和MDA、TNF-α、IL-1β浓度明显低于IR组,SPC组SOD活性和HO-1蛋白含量明显高于IR组(P<0.05)。结论七氟醚后处理能抑制脑缺血-再灌注时诱发的小胶质细胞激活,减轻脑组织氧化应激及炎症反应,从而减轻脑缺血-再灌注损伤,发挥其脑保护作用。Objective To investigate the effects of sevoflurane post-conditioning on oxidative stress and inflammatory reaction during rat cerebral ischemia-reperfusion,and to explore its cerebral protective mechanism.Methods Thirty-six health male Sprague-Dawley rats（aged 12-14 weeks,weighing 220-260g）were randomly divided into 3 groups（n=12 each）：sham control group（group Sham）,cerebral ischemia-reperfusion group（group IR）,sevoflurane post-conditioning group（group SPC）.Cerebral ischemia-reperfusion model was established,ischemia for 30 min followed by reperfusion 24 h.Rat middle cerebral artery was not occluded in group Sham.Cerebral ischemia-reperfusion model was established in group IR.Group SPC was subjected to 2.6% sevoflurane for 15 min in the beginning of reperfusion.At the end of reperfusion,rats were cut off the head to take out the brain tissue.The expression level of Iba-1and HO-1proteins was measured by western blot.The levels of reactive oxygen species（ROS）,malondialdehyde（MDA）,TNF-α,IL-1βand the activity of superoxide dismutase（SOD）were evaluated.Results Compared with group Sham,the expression of cerebral cortex Iba-1protein was higher than that in groups IR and SPC（P〈0.05）,the expression of Iba-1 protein in group SPC was lower than that in group IR（P〈0.05）.Compared with group Sham,the contents of ROS,MDA,TNF-αand IL-1β were increased in groups IR and SPC（P〈0.05）,but the activity of SOD and expression of HO-1protein were decreased（P〈0.05）.And the contents of ROS,MDA,TNF-αand IL-1β in group SPC were less than those in group IR,the activity of SOD and expression of HO-1protein in group SPC were higher than those in group IR.Conclusion Sevoflurane post-conditioning can mitigate the microglia activation,reduce cerebral oxidative stress and inflammation,thus protect rat cerebral against ischemia reperfusion injury.

关 键 词：脑缺血-再灌注 吸入麻醉 后处理 氧化应激 炎症 

分 类 号：R614[医药卫生—麻醉学]