环氧合酶-2、前列腺素E_2在百草枯诱导肺纤维化大鼠中的表达  

Expression of cyclooxygenase 2 and prostaglandin E_2 in rats with paraquat induced pulmonary fibrosis

作　　者：潘东峰[1,2] 梁沛枫[2,3] 肖红燕[2,4] 梁诗颂[1,2] 

机构地区：[1]宁夏人民医院急诊科,宁夏银川750002 [2]西北民族大学第一附属医院,宁夏银川750002 [3]宁夏人民医院统计室,宁夏银川750002 [4]宁夏人民医院病理科,宁夏银川750002

出　　处：《宁夏医学杂志》2017年第7期588-590,I0001,共4页Ningxia Medical Journal

基　　金：宁夏自然科学基金项目(NZ14161)

摘　　要：目的观察百草枯诱导的肺纤维化大鼠中环氧合酶-2(COX-2)、前列腺素E_2(PGE_2)的表达水平。方法 24只SD大鼠随机分为实验组和对照组各12只,实验组给予百草枯(50 mg/kg)一次性腹腔注射。HE染色观察染毒后28 d肺组织病理改变,双抗体夹心法测定大鼠血清、肺泡灌洗液(BALF)中COX-2、PGE_2水平,免疫组化检测肺组织中COX-2、PGE_2表达。结果与对照组相比,实验组染毒28 d后可见肺纤维改变,血清和BALF中COX-2表达水平均显著增高[(371.02±23.19)比(958.24±35.29),(351.09±22.54)比(943.68±33.17)pg/m L,P均<0.05);血清和BALF中PGE_2表达水平均显著增高[(110.56±12.30)比(366.23±17.54),(113.58±11.40)比(367.17±19.05)ng/L,P均<0.05)];肺组织COX-2和PGE_2阳性细胞数分级、阳性细胞显色强度分级和IHS评分均显著增高(COX-2:9.00±2.09比36.67±3.07,1.00±0.00比2.83±0.40,1.33±0.52比5.67±0.81;PGE_2:9.83±1.72比38.83±2.56,1.17±0.41比2.67±0.52,1.33±0.52比3.67±1.96,P均<0.05)。结论 COX-2、PGE_2在百草枯诱导的肺纤维化大鼠中高表达,参与了肺纤维的病理过程。Objective To observe the expression of cyclooxygenase 2 （ COX - 2） and prostaglandin E2 （ PGE2 ） in rats with pa- raquat - induced pulmonary fibrosis. Methods 24 SD rats were randomly divided into the experimental group and control group （ n = 12 ） , and the rats in the experimental group were given paraquat （50 mg/kg） by intraperitoneal injection for once. The pathology changes of lung tissue were evaluated by HE staining,the expression of COX- 2 and PGE2 in serum and alveolar lavage fluid （BALF） were de- tected by the double antibody clamp method, the expression of COX - 2 and PGE2 in lung tissue were detected by using the immunohisto- chemical method. Results Compared with the control group, pulmonary fibrosis changes were found in the experimental group after 28 days,the COX -2 expression in serum and BALF were significantly increased （371.02±23.19 vs. 958.24 ±35.29,351.09±22.54 vs. 943.68± 33.17 ） pg/ml （P 〈0.05 ） ;and PGE2 in serum and BALF expression levels were significantly higher （110.56 ±12.30 vs. 366.23 ± 17.54,113.58± 11.40 vs. 367.17±19.05 ng/L） （ P 〈 0.05 ） ; COX - 2 of lung tissue and PGE2 positive cells number classification, positive color intensity scale and IHS scores were significantly higher （ COX - 2 ： 9.00 ±2.09 vs. 36.67±3.07,1.00± 0.00vs. 2.83± 0.40,1.33 ±0.52vs. 5.67±0.81mm;PGE2：9.83±1.72vs. 38.83±2.56,1.17± 0.41vs. 2.67±0.52,1.33± 0.52 vs. 3.67 ±1.96） （ P 〈 0.05 ）. Conclusion COX - 2 and PGE2 are higher expression in rats with pulmonary fibrosis induced by paraquat ,they are participating in the pathological process of pulmonary fibrosis.

关 键 词：百草枯 环氧合酶-2 前列腺素E2 肺纤维化 

分 类 号：R563[医药卫生—呼吸系统] R595.4[医药卫生—内科学]