关键时间点神经再生条件液特异蛋白分析  

ANALYSIS OF SPECIFIC PROTEINS IN NERVE REGENERATION CONDITIONED FLUID AT DIFFERENT CRITICAL TIME POINTS

作　　者：李广帅[1] 李青峰[2] 董明敏[1] 丁爽[3] 郑岩[2] 朱旭冉 

机构地区：[1]郑州大学第一附属医院整形外科,河南郑州450052 [2]上海交通大学附属第九人民医院整复外科 [3]哈尔滨医科大学附属第一医院整形美容中心

出　　处：《齐鲁医学杂志》2017年第2期178-181,共4页Medical Journal of Qilu

基　　金：国家自然科学基金资助项目(30925034;81101437)

摘　　要：目的探讨周围神经再生关键时间点神经再生条件液(NRCF)的特异蛋白及其与不同时期周围神经再生的关系。方法建立周围神经再生室(NRC)模型,提取坐骨神经离断3、7、14和25d的NRCF标本,采用SDS-PAGE电泳、iTRAQ蛋白组学技术和质谱鉴定方法,进行蛋白定性和定量分析。结果坐骨神经离断各时间点NRCF样品蛋白质条带分布较均一,平行度良好,共鉴定出876种蛋白,其中坐骨神经离断3、7、14和25d的NRCF中独特蛋白质分别为95、137、18和58种。结论 NRCF蛋白含量丰富,种类繁多,每个关键时间点的特异蛋白与周围神经再生关系密切。Objective To investigate the specific proteins in the nerve regeneration conditioned fluid （NRCF） at four cri- tical time points and its relationship with the peripheral nerve regeneration in different stages of nerve injury repair. Methods A peripheral nerve regeneration chamber （NRC） model was established and the NRCF samples were collected at 3,7,14, and 25 d after transection of the sciatic nerve. The NRCF samples were analyzed using SDS-PAGE electrophoresis, isobaric tags for relative and absolute quantitation, and mass spectrometry. Results The protein bands of NRCF samples at the four time points were uniform and present with good parallelism. A total of 876 proteins were identified and the numbers of specific proteins were 95, 137,18, and 58, respectively, at 3,7,14, and 25 d after transection of the sciatic nerve. Conclusion There are rich and many types of proteins in the NRCF. The specific proteins at each critical time point are closely related to peripheral nerve regeneration.

关 键 词：神经再生条件液 周围神经损伤 蛋白组学 蛋白 

分 类 号：R338[医药卫生—人体生理学]