MTT法检测通络止痛方对人膝骨关节炎滑膜成纤维细胞增殖的影响  

Effect of Tongluo Zhitong Decoction on Proliferation of Synovial Fibroblasts in Patients with Knee Osteoarthritis by MTT

作　　者：杨黎黎[1] 王庆甫[1] 王欢[1] 张栋[1] 王伟利[1] 丁昊彬 甘稳 徐铭康 杜汪洋[1] 郭玉茹[1] 

机构地区：[1]北京中医药大学第三附属医院,北京100029

出　　处：《世界中医药》2017年第5期1105-1108,共4页World Chinese Medicine

基　　金：国家自然科学基金面上项目(编号:81373662);北京中医药大学自主选题项目(编号:2016-JYB-XS192)

摘　　要：目的:探讨通络止痛方对人膝关节骨性关节炎(Knee Osteoarthritis,KOA)滑膜成纤维细胞增殖的影响。方法:取本院行全膝关节置换术患者术中废弃滑膜组织,经I型胶原酶消化后进行原代培养,取3~4代成纤维细胞进行高、中、低浓度通络止痛方干预。应用四甲基偶氮唑盐法测定(MTT)法检测KOA滑膜细胞的增殖水平,并用倒置显微镜下观察各浓度下的细胞形态。结果:加药组组间与加药组与空白组比较,差异有统计学意义(P<0.05)。MTT法检测随通络止痛方浓度减小KOA滑膜细胞在490 nm波长处吸光OD值数越大;高中浓度组抑制率为正值,随浓度增加抑制率增大,低浓度抑制率为负值,随浓度降低抑制率减少。结论:高、中浓度通络止痛方对滑膜细胞增殖有抑制作用,低浓度组的通络止痛方对滑膜细胞有增殖有促进作用。Objective：To explore the effect of Tongluo Zhitong Decoction on the proliferation of knee osteoarthritis （KOA） synovial fibroblasts.Methods：The abandoned synovial tissues were taken from patients who had knee replacement surgery in the hospital.The tissues were primarily cultured after digestion in I type collagenase.Three to four generation fibroblasts were taken for high-medium-low concentration intervention.The proliferation of KOA synovial cells was detected by 4-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide （MTT） and the cell morphology was observed under the inverted microscope.Results：Comparisons between the drug groups and comparisons between the drug groups and the control group,the difference was statistically significant （P〈0.05）.MTT assay showed that s the light OD number of KOA synovial cells became greatest in the wavelength of 490 nm when Tongluo Zhitong Decoction concentration decreased.The inhibition rates of high-medium concentration groups were positive.With the increase of the concentration,the inhibition rate increased and the inhibition rate of the low concentration group was negative.With the concentration decreased,the inhibition rate reduced.Conclusion：The medium-high concentrated Tongluo Zhitong Decoction may inhibit the proliferation of synovial cells,while low concentrated Tongluo Zhitong Decoction may promote the proliferation of synovial cells.

关 键 词：通络止痛方 MTT法检测 人膝骨关节炎 成纤维细胞 

分 类 号：R274.9[医药卫生—中西医结合] R332[医药卫生—中医骨伤科学]