稳心丹对MIRI大鼠相关炎性因子的影响  

Effect of Wen Xin Dan on Inflammatory Factors of MIRI Rats

作　　者：沈晓璞[1,2] 李秀典[2] 李冀[1] 

机构地区：[1]黑龙江中医药大学,哈尔滨150040 [2]黑龙江中医药大学附属第二医院,哈尔滨150001

出　　处：《世界中医药》2017年第5期1128-1133,共6页World Chinese Medicine

基　　金：黑龙江省中医药科研项目(编号:ZHY16-024);黑龙江中医药大学校基金(编号:201405)

摘　　要：目的:观察稳心丹对MIRI大鼠心肌酶指标CK-MB、c Tn I以及肿瘤坏死因子-α(TNF-α)水平、白介素-6(IL-6)的影响,探讨稳心丹对MIRI大鼠相关炎性因子的影响。方法:将70只SD大鼠随机分成随机分7组,每组10只,分别为假手术组、模型组、预处理组、稳心丹1∶1∶1组、稳心丹1∶2∶1组、稳心丹1∶1∶2组、稳心丹2∶1∶1组。灌胃14 d后,以结扎大鼠冠状动脉左前降支(LAD)后再复灌建立心肌缺血再灌注模型。全自动生化仪测定CK-MB含量,ELISA法检测c Tn I、TNF-α、IL-6含量。结果:模型组大鼠血清中CK-MB、c Tn I含量明显升高,与假手术组比较差异有统计学意义(P<0.01);与模型组比较,各观察组的心肌酶指标都有改变,差异有统计学意义(P<0.01),其中1∶2∶1组CK-MB下降最明显;模型组大鼠血清中TNF-α、IL-6含量明显升高,与假手术组比较差异有统计学意义(P<0.01);与模型组比较,各观察组的TNF-α、IL-6含量都有改变,差异有统计学意义(P<0.01),其中1∶2∶1组TNF-α、IL-6下降最明显。结论:稳心丹可降低MIRI大鼠血清CK-MB、c Tn I以及TNF-α、IL-6含量而产生抗炎作用。To observe the effect of Wen Xin Dan on myocardial enzyme indicators, including CK-MB, cTnI and tumor necrosis factor alpha （TNF alpha） levels, and interleukin 6 （IL-6） of MIRI rats.Besides, to explore its effect on inflammatory factor of MIRI rats.Methods：A total of 70 SD rats were randomly divided into 7 groups with 10 in each.Groups include control group, model group, pretreatment group, Wen Xin Dan1∶1∶1 group, Wen Xin Dan1∶2∶1 group, Wen Xin Dan1∶1∶2 group, Wen Xin Dan2∶1∶1 group.After 14 days lavage, ligate rat′s left anterior descending coronary artery （LAD）.Again, lavage the rats to make myocardial ischemia models.Use automatic biochemical instrument to measure CK-MB content and cTnI ELISA method to measure TNF alpha, IL-6 levels.Results：Serum CK-MB, cTnI levels increased significantly in model group with significant difference comparing to those of the control group （P〈0.01）.Compared to the model group, myocardial enzyme index in each Wen Xin Dan group has changed, and the difference is significant （P〈0.01）, among which, CK-MB in the 1∶2∶1 group reduced most significantly.Serum TNF alpha, IL-6 levels increased significantly in the model group with significant difference especially compared to those of the control group （P〈0.01）.Compared with model group, TNF alpha and IL-6 content has changed significantly in all Wen Xin Dan group （P〈0.01）, among which the 1∶2∶1 group has the largest decrease.Conclusion：Wen Xin Dan can reduce serum CK-MB, cTnI and the content of TNF alpha, IL-6, therefore, it has anti-inflammatory effects.

关 键 词：稳心丹 心肌缺血再灌注 TNF-α、IL-6 

分 类 号：R285.5[医药卫生—中药学]