阿托伐他汀对兔急性心肌梗死后心肌miR-221/miR-222表达及血管新生的影响  

Effect of atorvastatin on angiogenesis and expression of miR-221/miR-222 in rabbits with acute myocardial infarction

作　　者：张夏晓 谈红[1] 陈瑞敏[1] 苏莉[2] 晋群[1] 

机构地区：[1]济南军区总医院心内科,济南250031 [2]济南军区总医院干部三科

出　　处：《临床心血管病杂志》2017年第7期699-703,共5页Journal of Clinical Cardiology

基　　金：济南军区总医院院长基金(No:2013MS05)

摘　　要：目的:探讨阿托伐他汀对兔急性心肌梗死后梗死心肌组织miR-221/miR-222表达以及血管新生的影响。方法:40只新西兰大白兔随机选取8只设为假手术组,只在左冠状动脉前降支下穿线不结扎,剩余32只兔建立急性心肌梗死模型后随机均分为2组:模型组和他汀组。他汀组给予阿托伐他汀钙片1mg·kg-1·d-1,连续灌胃4周;假手术组和模型组给予等量助溶剂连续灌胃4周。4周后处死,取梗死心肌组织,采用免疫组织化学法检测梗死区心肌组织微血管密度(MVD),采用RT-PCR法检测miR-221/miR-222的表达水平。结果:与模型组MVD(3.450±1.036)相比,他汀组(7.640±1.804)明显增加(P<0.05)。与假手术组miR-221/miR-222表达水平(0.716±0.083/0.692±0.069)比较,模型组miR-221/miR-222表达水平(1.010±0.043/1.009±0.042)明显升高,他汀组(0.431±0.111/0.374±0.118)明显降低(P<0.05)。他汀组梗死组织MVD和miR-221/miR-222的表达水平呈显著负相关(r=-0.755,P<0.01;r=-0.804,P<0.01)。结论:阿托伐他汀可能通过抑制miR-221/miR-222的表达促进心肌梗死后梗死心肌组织的血管新生。Objective：To investigate the effect of atorvastatin on angiogenesis and expression of miR-221/miR-222 after acute myocardial infarction in rabbits.Method：Eight New Zealand white rabbits were randomly selected from a total of40 rabbits as sham operated group,were only threaded under the left anterior descending coronary artery without ligation.The remaining 32 rabbits to establish acute myocardial infarction model were randomly divided into the model group and the statin group.Then the rabbits in the statin group were given atorvastatin at 1 mg·kg-1·d-1 by gavage for 4weeks,and those in the model group and sham operation group were given equivalent cosolvent.After 4weeks,the rabbits were executed and the myocardial infarction samples were prepared.The myocardial microvessel density was detected by immunohistochemical staining,and the expression level of miR-221/miR-222 was determined by RT-PCR.Result：Compared with the model group（3.450±1.036）,the MVD in the statin group（7.640±1.804）increased significantly（P0.05）.Compared with the sham operation group（0.716±0.083/0.692±0.069）,the expression level of miR-221/miR-222 in the model group（1.010±0.043/1.009±0.042）was significantly increased,which was significantly decreased in the statin group（0.431±0.111/0.374±0.118）,P0.05.The microvessel density and the expression level of miR-221/miR-222 were negatively related in myocardial infarction tissue of stain group（r=-0.755,P0.01;r=-0.804,P0.01）.Conclusion：Atorvastain may promote angiogenesis in infarction myocardium by deprssing the expression of miR-221/miR-222.

关 键 词：急性心肌梗死 阿托伐他汀 miR-221/miR-222 血管新生 

分 类 号：R542.2[医药卫生—心血管疾病]