黄芪汤对人体代谢的影响及其在疾病防治中的辅助作用  

Influence of Huangqi Decoction on Human Metabolism and Its Auxiliary Role in Disease Prevention and Treatment

作　　者：王守丽[1] 谢国祥[1] 赵爱华[1] 陈天璐[1] 贾伟[1] 

机构地区：[1]上海交通大学附属第六人民医院转化医学中心,200233

出　　处：《医学研究杂志》2017年第8期26-30,共5页Journal of Medical Research

基　　金：国家自然科学基金资助项目(31501079)

摘　　要：目的本研究采用代谢组学技术和方法,研究健康成人口服黄芪汤前后血浆代谢谱的变化,为黄芪汤的药效研究提供依据和参考。方法联合采用气相色谱质谱和液相色谱质谱两个平台,结合单变量和多变量统计分析方法,考察服用黄芪汤前后人体血浆中内源性代谢物的波动情况。结果主成分分析和正交偏最小二乘法判别分析结果均显示,服药前和服药后2h,人体血浆代谢谱有明显的分离趋势,内源性代谢物发生了显著的波动。借助单维和多维统计分析方法,筛选出了49种服药前后的差异代谢物。黄芪汤中的主要成分芒柄花素及其代谢产物芒柄花素葡萄糖苷的水平在服药后均显著升高。色氨酸、苯丙氨酸和胆酸等物质显著降低。结论服用黄芪汤后人体氨基酸代谢、脂肪代谢、糖类代谢和胆酸代谢均有波动,这些代谢物与肝病和糖尿病的防治有紧密关联。Objective To investigate the changes of metabolic profiles in healthy adults before and after oral administration of HQD, and to provide new insights for the pharmacodynamic study of HQD. Methods Gas chromatography time - of - flight mass spectrometry （ GC - TOFMS） and ultra - high performance liquid chromatography quadrupole time of flight mass spectrometry （ UPLC - QTOFMS） plat- forms, combined with univariate and multivariate statistical analysis, were used to investigate the fluctuations of endogenous metabolites in pre - and post - dose plasma samples of healthy individuals. Results Principal component analysis （PCA） and orthogonal partial least squares diseriminant analysis （ OPLS - DA） consistently showed that the separation trends of metabolic profiles derived from pre and post- dose groups were highly significant, which indicated that the endogenous metabolites changed greatly after taking HQD. Further more, forty - nine differential metabolites were screened out by using univariate and multivariate statistical analysis. Comparing with pre - dose group, formononetin （the principal component of HQD） and its downstream product formononetin glucuronide were both higher whereas tryptophan, phenylalanine and cholic acid were lower in post- dose group. Conclusion Amino acid, lipid and fatty acid, glu- cose and cholic acid metabolism were all altered after taking HQD. These metabolites are closely associated with the prevention and thera- py of liver diseases and diabetes.

关 键 词：黄芪汤 代谢组学 肝病 糖尿病 

分 类 号：R49[医药卫生—康复医学]