RP-HPLC法测定人血浆中卡马西平浓度  

Determination of the Concentration of Carbamazepine in Human Plasma by RP-HPLC with UV Detection

作　　者：王丽霞[1] 陈清霞[2] 杨红梅 梁小丽 刘伟忠[1] 

机构地区：[1]广州医科大学附属脑科医院(广州市惠爱医院)药学部,广州510370 [2]中山大学附属第二医院药学部,广州510120 [3]广州市妇女儿童医疗中心药学部,广州510120

出　　处：《海峡药学》2017年第7期52-55,共4页Strait Pharmaceutical Journal

摘　　要：目的建立反相高效液相色谱法测定人血浆中卡马西平浓度的方法。方法以Agilent TC-C18反相柱(150mm×4.6mm,5μm)为色谱柱,流动相为0.03mol·L^(-1)醋酸铵-乙腈-甲醇(20∶15∶65 V/V/V);流速:0.8m L·min-1;检测柱温:35℃;检测波长:285nm。灵敏度为0.01AUFS。以乙酸乙酯和二氯甲烷(80∶20 V/V)为提取剂。结果卡马西平的低、中、高(2.0,8.0,48.0μg·m L^(-1))3种浓度平均相对回收率分别为101.1%,100.6%,99.89%,提取回收率分别为81.91%,85.02%,88.53%;日内、日间相对标准偏差(RSD)均低于10%(n=5);分析方法的检测限0.5μg·m L^(-1);线性范围为1.0~64.0μg·m L^(-1)。线性方程:Y=12.512X+1.46,相关系数r=0.9991(n=8)。结论该方法简单、快速、灵敏、准确,可用于卡马西平临床血药浓度监测和药动学研究。OBJECTIVE To establish a method for determining the concentration of carbamazepine in human plasma by RP-HPLC. METHODS The drug from plasma was analyzed in a reverse phase HPLC system Agilent TC-C18 column （ 150 mm×4.6mm,5 μm） ; mobile phase consisted of 0. 03 mol ·L^-1 ammonioum-acetonitrile-methanol（20：15：65v/v/v） ;the flow rate was 0. 8mL·min^-1; the detection temperature was at 35℃, the detection wave- length was at 285nm. The sensitivity was 0. 01 AUFS. Ethyl acetate and dichloromethane was used as extracting sol- vent. RESULTS The average recoveries of carbamazepine in low, middle and high concentrations （2. 0,8.0,48.0 μg·m L^-1 ） were 101.3 % , 100. 6% and 99.89% , respectively, the extraction recovery were81.91% , 85.02% and 88.53 % , respectively. The intra-day and inter-day variation （RSD） was less than 10% （ n = 5 ）. The calibration curve of earbamazepine showed good linearity, r = 0. 9991 （ n = 8 ）, over the range of 1.0 - 64.0μg·m L^-1. The mini- mum detectable concentration of carbamazepine was 0. 5μg·m L^-1. The regression equation was Y = 12. 512X ＋ 1.46. CONCLUSION The method is sensitive, quick, simple and accurate, it can be used for clinical drug monito- ring and pharmacokinetics studies of carbamazepine.

关 键 词：卡马西平 血药浓度 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]