鸭大肠杆菌血清型分析和疫苗菌株的筛选  

Serotypes of duck Escherichia coli and strain selection for vaccine

作　　者：袁小远[1] 王晓丽 亓丽红[1] 邵桂宾 邵桂平 杨金兴[1] 艾武[1] 王永明 

机构地区：[1]山东省农业科学院家禽研究所,济南250023 [2]山东华宏生物工程有限公司,滨州256600 [3]山东莒县众鑫农牧有限公司,日照276500

出　　处：《中国人兽共患病学报》2017年第7期604-606,共3页Chinese Journal of Zoonoses

基　　金：山东省自然科学基金项目(No.ZR2015CM009);科技基础性工作专项(No.2012FY111000);山东省2015年度农业重大应用技术创新项目;山东省农业科学院院青年基金项目(No.2014QNM15;No.2015YQN61;No.2016YQN56)联合资助~~

摘　　要：目的分析鸭大肠杆菌的血清型分布,并进行疫苗菌株的筛选。方法近年来从山东、河北等地区的商品鸭场送检的病料中分离大肠杆菌,并进行血清型鉴定和生物特性分析;通过毒力检测和免疫原性试验筛选疫苗菌株。结果共分离到44株鸭大肠杆菌。根据菌体抗原O进行分型,共鉴定血清型有6种:O78、O93、O76、O2、O92和O32;其中O78为优势血清型,占56.8%(25/44);O93血清型次之,占15.9%(7/44)。免疫原性试验证实O78血清型菌株SD具有较强的毒力和良好的免疫原性。结论分析地区的鸭场鸭大肠杆菌以O78、O93、O76为优势血清型;免疫原性较好的SD菌株可作为下一步研制灭活疫苗的候选菌株。In order to obtain the serotype distribution of E.coli from duck and to screen the vaccine bacterial strains,the serotype identifications and biological characteristics of E.coli were analyzed in recent years from Shandong,Hebei and other areas of commercial duck field;selections of vaccine strains were detected by the virulence and immunogenicity.Totally 44 isolated bacterial strains of E.coli from duck were identified to a total of six serotypes：O78,O93,O76,O2,O92 and O32.The O78 serotype was the dominant serotype,accounting for 56.8%（25/44）;O93 serotype for 15.9%（7/44）according to bacterial Oantigen typing.The strain SD（O78 serotype）was confirmed to have strong virulence and good immunogenicity.The O78,O93 and O76 are the dominant serotypes of duck E.coliin the study areas.The SD strain could be used as the candidate for the next development of inactivated vaccine.

关 键 词：鸭大肠杆菌 血清型 生物学特性 疫苗菌株 

分 类 号：R378.2[医药卫生—病原生物学]