福建省鼠类感染巴尔通体调查及序列分析  

Investigation and sequence analysis on Bartonellainfection in rodents in Fujian Province,China

作　　者：肖方震[1] 林代华[1] 周淑姮[1] 徐国英[1] 邓艳琴[1,2] 

机构地区：[1]福建省疾病预防控制中心福建省人兽共患病研究重点实验室,福州350001 [2]福建医科大学,福州350004

出　　处：《中国人兽共患病学报》2017年第7期607-612,共6页Chinese Journal of Zoonoses

基　　金：福建省医学创新课题(No.2012-CXB-12);福建省自然科学基金项目(No.2017J01139)联合资助~~

摘　　要：目的了解福建省鼠类中巴尔通体(Bartonellaspp.)的感染状况和基因特征。方法 2014-2016年采用笼日法在福建省闽东、闽西、闽南、闽北和闽中捕鼠,现场鉴定并记录捕获鼠类的釆集时间、地点、鼠种、性别、鼠龄等资料。采集鼠心脏血,PCR扩增巴尔通体的gltA和16S^23SrRNA基因,阳性PCR产物送测序并进行序列比对分析,构建系统进化树。感染率间的比较采用χ~2检验或Fisher精确检验法。结果调查共布放鼠笼5 917笼次,捕鼠381只,鼠密度为6.44%。巴尔通体感染率为12.34%。家鼠的巴尔通体感染率为10.61%,褐家鼠(Rattus norvebicus)和黄胸鼠(Rattus flavipectus)感染率分别为11.30%和10.00%;野鼠的感染率为13.86%,黄毛鼠(Rattus losea)和针毛鼠(Rattus fulvescens)感染率分别为22.86%和18.00%,野鼠的巴尔通体感染率高于家鼠的感染率,但无统计学意义。从地区分布看,闽西、闽北一带的感染率较高,分别为20.00%和25.33%。闽南一带感染率最低,为0。各地区感染率存在统计学意义(P<0.05)。不同性别、鼠龄的巴尔通体感染率差异无统计学意义,而不同的生境下的感染率存在统计学差异(P<0.05)。阳性样本测序分析显示,福建省鼠类感染的巴尔通体序列与B.tribocorum、B.elizabethae和B.grahamii序列最接近。结论福建省鼠类存在巴尔通体感染,存在对人群致病的风险。We explored the status of Bartonellainfection in rodents and the sequence characteristics of Bartonellain Fujian Province.Rodents in Fujian Province were captured by the night trapping method during 2014-2016.Information of the captured rodents on capturing dates and geographic locations,species,gender and ages were recorded.Heart blood samples were collected,from which the fragments of gltA gene and 16S-23S rRNA gene of Bartonella were amplified by polymerase chain reaction（PCR）.The PCR products were sequenced and the phylogenetic tree was constructed for homology analysis by biological analysis software.Data on infection rate were analyzed with Chi-square or Fisher exact test to indicate statistical significance.Results showed that 5 917 cages were laid and 381 rodents were captured,density of rodent was 6.44%.The overall Bartonel lainfection rate in rodents was 12.34%,while infection rate in domesticated rodents was 10.61%,with 11.30% in Rattus norvebicus and 10.00% in R.flavipectus.And the infection rate in wide rodents was 13.86%,with a rate of 22.86% in Rattus losea and 18.00% in R.fulvescens,respectively.The infection rate was higher in wild rodents than in domesticated rodents,however,no significant difference was found.The Western Fujian and Northern Fujian region had the higher infection rates of 20.00% and 25.33%,and no infection was found in Southern Fujian region.The statistical analysis result revealed that a significant difference in infection rate among different region and habitats,but no significant difference in infection rate between male and female rodents,or among different ages.The BLAST results revealed the species to be B.tribocorum,B.elizabethae and B.grahamii.In conclusion,Bartonellainfection is found in the rodents in Fujian Province and more attention should be paid on its impact on public health in the province.

关 键 词：巴尔通体 鼠类 基因特征 福建 

分 类 号：R37[医药卫生—病原生物学]