五倍子酸对人胃癌SGC-7901细胞迁移能力的影响  

Influence of gallic acid in migration ability of human gastric carcinoma SGC-7901 cells

作　　者：王丽萍[1] 冯若[1] 邹立洋 丁一[1] 

机构地区：[1]郑州大学基础医学院组织学与胚胎学教研室,河南郑州450001 [2]解放军第301医院输血科,北京100853

出　　处：《吉林大学学报（医学版）》2017年第4期739-742,I0004,F0003,共6页Journal of Jilin University：Medicine Edition

基　　金：河南省科技厅科技攻关项目资助课题(122102310233);河南省卫计委科研项目资助课题(200803061)

摘　　要：目的:探讨五倍子酸(GA)对人胃癌SGC-7901细胞转移的影响,阐明其作用机制。方法:培养人胃癌SGC-7901细胞,将其分为对照组及3.125、6.250、12.500、25.000、50.000和100.000mg·L^(-1) GA组。MTT法检测各组SGC-7901细胞增殖抑制率,细胞划痕实验检测各组细胞转移能力,免疫细胞化学法检测各组SGC-7901细胞中血管内皮生长因子(VEGF)表达水平。结果:与对照组比较,不同剂量GA组SGC-7901细胞增殖抑制率明显升高,并呈剂量依赖性(F=59.451,P<0.01)。与对照组比较,不同剂量GA组SGC-7901细胞的划痕愈合率明显降低(P<0.01),12.500和25.000mg·L^(-1) GA组细胞中VEGF蛋白表达水平明显降低(P<0.05)。结论:GA通过抑制人胃癌SGC-7901细胞中VEGF蛋白的表达来抑制SGC-7901细胞增殖及迁移。Objective： To study the influence of gallic acid （GA） on the migration of human gastric carcinoma SGC-7901 cells, and to explore its mechanism. Methods： The human gastric carcinoma SGC-7901 ceils were cultured and divided into control group and 3. 125, 6. 250, 12. 500, 25. 000, 50. 000, 100. 000 mg · L^-1 GA groups. The inhibitory rates of proliferation of SGC-7901 cells in various groups were examined by MTT assay; the migration abilities of SGC-7901 cells in various groups were measured with scratch assay; the expression levels of vascular of endothelial growth factor （VEGF） in various groups were detected by immunocytochemistry. Ftesults： Compared with control group, the inhibitory rates of proliferation of SGC-7901 cells in different doses of GA groups were significantly increased in a dose-dependent manner （F=59. 451, P〈0. 01 ）. Compared with control group, the wound healing rates in different doses of GA groups were significantly decreased （P〈0. Ol）. Compared with control group, the expression levels of VEGF protein in 12. 500 and 25. 000 mg · L^-1 GA groups were decreased （P（ 0.05）. Gonclusion.. GA could inhibit the proliferation and migration of SGC-7901 cells through down-regulating the expression levels of VEGF protein.

关 键 词：五倍子酸 SGC-7901细胞 细胞迁移 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]