奶牛乳房炎大肠杆菌的青霉素耐药特征研究  

Penicillin-resistant Characterization of Escherichia coli Isolated from Bovine Mastitis

作　　者：张莉莉[1,2] 杨峰[1] 李宏胜[1] 王益民[2] 刘龙海[1] 张哲[1] 王丹[1] 张亚茹[1] 李新圃[1] 罗金印[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点实验室甘肃省新兽药工程重点实验室,兰州730050 [2]兰州大学公共卫生学院,兰州730000

出　　处：《中国草食动物科学》2017年第4期38-41,共4页China Herbivore Science

基　　金：国家自然科学基金项目(81402703;81550046)

摘　　要：为了研究奶牛乳房炎大肠杆菌的青霉素耐药特征,采用药敏纸片法检测了123株大肠杆菌对青霉素的耐药性,并利用β-内酰胺酶试剂盒测定耐青霉素株β-内酰胺酶活性,同时采用刚果红法和改良结晶紫半定量方法检测生物被膜的形成能力。结果表明:123株受试大肠杆菌中,对青霉素耐药的菌株有122株,耐药率99.19%。122株耐青霉素大肠杆菌中,生物被膜检测为阳性的菌株有92株(75.41%),β-内酰胺酶检测为阳性的有72株(59.02%);β-内酰胺酶阳性而生物被膜阴性的菌株有15株(12.30%),β-内酰胺酶和生物被膜同为阳性的有57株(46.72%);β-内酰胺酶为阴性但生物被膜为阳性的菌株有35株(28.69%)。92株生物被膜阳性菌株中,29株(31.52%)具有强成膜能力,61株(66.30%)具有中等成膜能力,2株(2.17%)成膜能力较弱。说明奶牛乳房炎大肠杆菌对青霉素的耐药率较高;大肠杆菌青霉素耐药性受β-内酰胺酶和生物被膜多种因子的共同调控。The aim of this study was to investigate the penicillin resistant characterization of Escherichia coli isolated from bovine mastitis. The penicillin resistance was determined by disk diffusion method, β-1actamase test kit was used to detect the β-1actamase activity, biofilm formation was tested with Congo red Agar and semi-quantitative adherence assay method. The results showed that, in all tested E.coli, 122 isolates were resistant to penicillin, the resistance rate was 99.19%. Among the 122 penicillin-resistant E.coli strains , 72（ 59.02% ） isolates were identified as β-lactamase producers, and 92 （75.41% ） isolates produced biofilm. In addition, 15（ 12.30% ） biofilm-negative strain was identified as β-lactamase producer. Meanwhile, 57 （46.72%） isolates were positive for both of β-lactamase and biofilm, 35 （28.69%） β-lactamase-negative strains were identified as biofilm producers. The results of semi-quantitative adherence assay method showed that, among the 92 isolates bioflm producer, 29（ 31.52% ） were detected as strong biong producer, 61 （66.30%） were detected as moderate bioflm producer, and 2（ 2.17% ） were detected as weak bioflm producer.The study indicated that E. coli exhibited high resistance rates to penicillin, and implied that E. coli resistant to penicillin was attributed to multiple mechanisms.

关 键 词：奶牛乳房炎 大肠杆菌 青霉素 耐药性 Β-内酰胺酶 生物被膜 

分 类 号：S823.7[农业科学—畜牧学]