高效液相色谱法同时测定油菜秸秆水解液中的糠醛和5-羟甲基糠醛  

Simultaneous determination of furfural and 5-hydroxymethylfurfural in the hydrolysate of rape straw by high performance liquid chromatography

作　　者：冯广荣 鲁厚芳[1,2] 梁斌[1,2] 刘颖颖[2] 

机构地区：[1]四川大学化学工程学院,四川成都610065 [2]四川大学新能源与低碳技术研究院,四川成都610065

出　　处：《可再生能源》2017年第7期949-956,共8页Renewable Energy Resources

基　　金：国家自然科学基金项目(21336008)

摘　　要：生物质水解液中组分的确定可为生物质水解历程分析、组分后续分离与应用提供依据。文章建立了生物质水解液中糖的降解产物糠醛(F)和5-羟甲基糠醛(5-HMF)的高效液相色谱(HPLC)分析方法。该分析方法采用Alltima^(TM) C_(18)色谱柱,柱温为30℃;紫外检测器(UV),检测波长为285 nm;流动相为体积比为10∶90的乙腈和水(含0.1%的乙酸),流速为1.0 m L/min,等度洗脱。通过该分析方法,F和5-HMF能够得到很好地分离,其线性回归方程的相关系数R2分别为0.999 8和0.999 9,平均回收率分别为99.8%和100.3%,相对标准偏差RSD均小于1%。此液相分析方法精密度、准确度高,重现性好,为生物质水解液中糖的降解产物F和5-HMF含量的测定提供了一种准确、灵敏、快速的分析方法。To analyze the hydrolysis process, separate and make use of the components in straw hydrolysate, it is important to determine them. In this work, furfural（F） and 5-hydroxymethylfurfural（5-HMF） which are the degradation products of monosaccharides in biomass hydrolysate were detected by high performance liquid chromatography（HPLC）. The samples were separated by Alltima^（TM） C_（18） column at 30 ℃. The mobile phase was acetonitrile and water（with 0.1%acetic acid） under a fixed volume ratio of 10 ∶90, and the flow rate was 1.0 m L/min. They were detected by ultraviolet（UV） detector at 285 nm. Excellent linearity can be obtained（R2： 0.999 8 for F and 0.999 9 for 5-HMF）. The average recovery values were 99.8% and 100.3% respectively, and the relative standard deviation percentage（RSD） was less than 1%. This method can achieve good precision, high accuracy and good reproducibility, and provide an accurate, sensitive and rapid analysis method for F and 5-HMF in straw hydrolysate.

关 键 词：高效液相色谱 秸秆水解液 糠醛 5-羟甲基糠醛 

分 类 号：TK6[动力工程及工程热物理—生物能] S216.2[农业科学—农业机械化工程]