肺痿论治干预肺纤维化大鼠肺功能的实验研究  

Experimental Study on Lung Function of Pulmonary Fibrosis Rat under Lung Withered Theory

作　　者：康荻 李彧[1] 朱曼[1] 唐磊[1] 申萌萌[1] 常立国[1] 黄春芳[1] 王继峰[1] 牛建昭[1] 李亚东[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《世界中医药》2017年第2期390-393,397,共5页World Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81202787;81573716);教育部国家外专局创新引智基地资助项目(编号:B07007)

摘　　要：目的:比较麦门冬汤和甘草干姜汤在肺纤维化阶段对大鼠模型肺功能的干预作用。方法:用博莱霉素造模14 d的SD大鼠,灌胃麦门冬汤(7.5 g/kg、15 g/kg、30 g/kg)和甘草干姜汤组(3.75 g/kg、7.5 g/kg、15 g/kg),模型组和正常组大鼠灌胃等体积饮用水。中药灌胃14 d后检测用力肺活量(FVC)、肺动态顺应性(Cdyn)、0.4秒率(FEV0.4/FVC%)、呼气峰值流速(PEF)、最大呼气中期流速(MMF)等指标,检测血气变化,称量脏器质量、计算脏器系数,HE染色观察肺组织病理。结果:麦门冬汤高剂量组能够降低模型动物肺脏质量、肺脏系数(P<0.05),提高肺顺应性、FVC和质量FVC(P<0.05);甘草干姜汤高剂量能够改善肺顺应性和FVC(P<0.05);甘草干姜汤中剂量能够改善质量FVC、降低0.4秒率(P<0.05),保护非纤维化区肺泡结构;甘草干姜汤能够提高肺纤维化大鼠的PO2和SO2(P<0.05)。结论:麦门冬汤和甘草干姜汤对博莱霉素所致肺纤维化大鼠肺脏功能均具有改善作用,前者主要是改善气道周围纤维化区的间质增生,后者主要是保护非纤维化区的肺泡结构和功能。Objective：To observe different effects of Maimendong Decotion and Gancao GanjiangDecotionin on pulmonary fibrosis rat in the stage of fibrotic phase.Methods：After 14 days of establishing rat model by intratracheal injection of bleomycin,Maimendong decotion（7.5 g/kg,15 g/kg,30 g/kg） and Gancao Ganjiang decotion（3.75 g/kg,7.5 g/kg,15 g/kg） were given by gavage once a day for 2 week.Meanwhile,the model group and the normal group were administered with distilled water of same quantity.After gavage of decotion for 14 days,the forced vital capacity （FVC）,the indexes of lung dynamic compliance（Cdyn）,the ratio of FEV 0.4 to forced vital capacity（FEV0.4/FVC%）,peak expiratory flow rate （PEF）,maximal mid-expiratory flow rate （MMF） and the blood gas changes were recorded,then the organ coefficients were calculated,lung tissue and airway pathologic changes were observed by HE staining.Results：Compared with the model group,the high dose group of Maimendong decotion reduced the lung weight and lung index significantly（P〈0.05）,and increased Cdyn,FVC and FVC/lung weight significantly（P〈0.05）.The Cdyn and FVC of high dose group of Gancao Ganjiang decotion were higher than the model group（P〈0.05）.The intermediate-dose Gancao Ganjiang decotion improved FVC/lung weight （P〈0.05） and reduced the rate of FEV0.4/FVC% significantly （P〈0.05） compared with the model group.Gancao Ganjiang decoction improved PO2 and SO2 significantly compared to model group（P〈0.05）.Conclusion：Both Maimendong decotion and Gancao Ganjiang decotion are better for the rat model of bleomycin induced pulmonary fibrosis.The former can reduce the interstitial hyperplasia around the airway in fibrosis area,and the latter protect the structure and function of the alveolar in non-fibrosis area.

关 键 词：甘草干姜汤 麦门冬汤 质量FVC 血气分析 肺顺应性 

分 类 号：R285.5[医药卫生—中药学]