清胰Ⅱ号颗粒剂对急性重症胰腺炎大鼠胰腺损伤的保护作用  

Protective Effects of Qingyi II Granules against Pancreas Injury in Rats with Severe Acute Pancreatitis

作　　者：肖青川[1] 兰天罡[2] 兑丹华[2] 

机构地区：[1]武警四川总队医院肝胆一外科,乐山614000 [2]遵义医学院附属医院肝胆胰外科,遵义563003

出　　处：《世界中医药》2017年第4期875-878,共4页World Chinese Medicine

基　　金：贵州省省长专项资金项目(编号:黔省专合字(2008)106号)

摘　　要：目的:观察清胰Ⅱ号颗粒剂对重症急性胰腺炎(Severe Acute Pancreatitis,SAP)大鼠胰腺损伤的保护作用并探讨其机制。方法:将18只SD大鼠随机等分为假手术组(A组)、SAP组(B组)、治疗组(C组)。以30 g/L牛磺胆酸钠逆行注入胰胆管制作大鼠SAP模型。C组以250 g/L清胰Ⅱ号颗粒剂灌胃给药(10 m L/kg),1次/6 h,A组、B组用同等剂量生理盐水以相同方式灌胃。制模后24 h取材,用real-time PCR检测胰腺组织中Hmgb1 mRNA表达,ELISA测定胰腺组织中一氧化氮和内皮素-1浓度,同时观察胰腺组织病理改变,测定胰腺组织湿/干重比值。结果:B组有胰腺组织损伤改变,Hmgb1 mRNA的表达上调,一氧化氮和内皮素-1浓度升高;清胰Ⅱ号颗粒剂可下调胰腺组织中Hmgb1mRNA的表达,降低一氧化氮及内皮素-1浓度,减轻胰腺病理损害程度。结论:Hmgb1、一氧化氮及内皮素-1在SAP胰腺损伤中可能具有重要作用,清胰Ⅱ号颗粒剂可能通过下调胰腺组织中Hmgb1 mRNA的表达,降低一氧化氮和内皮素-1浓度,减轻胰腺组织的病理改变,从而保护胰腺损伤。Objective：To observe the effect and mechanism of Qingyi II Granules （QY） against the pancreas injury in rats with severe acute pancreatitis （SAP）.Methods：Eighteen SD rats were randomly divided into fake operation group （group A）, SAP group （group B） and treatment group （group C）.The rat SAP model was made by retrograde injection of 30 g/L sodium taurocholate into pancreatic duct.Group C was treated with 250 g/L Qingyi Ⅱ Granule （10 mL/kg）, once per 6 h, and group A and group B were treated with the same dose of saline in the same way.The expression of Hmgb1 mRNA in pancreatic tissue was detected by real-time PCR after 24 hours of modeling.The concentration of nitric oxide and endothelin-1 in pancreas was measured by enzyme-linked immunosorbent assay （ELISA）.The pathological changes of pancreas were observed and the wet/dry weight ratio of pancreas was measured.Results：There were pancreas injury changes in group B, besides, expression of Hmgb1 mRNA was up-regulated and the levels of nitric oxide and endothelin-1 were increased;Qingyi Ⅱ Granule may cause that the expression of Hmgb1 mRNA in pancreatic tissue, the levels of nitric oxide and endothelin-1 concentration were decreased, thus reduced the degree of pathological damage of the pancreas.Conclusion：Hmgb1, nitric oxide and endothelin-1 may play an important role in SAP pancreatic injury.Qingyi II granules may reduce the expression of Hmgb1 mRNA in pancreatic tissue and decrease the concentration of nitric oxide and endothelin-1 to reduce the pathological changes of pancreatic tissue, thus protecting the pancreas injury.

关 键 词：高迁徙率族蛋白-1 内皮素-1 一氧化氮 重症急性胰腺炎 大鼠 

分 类 号：R285.5[医药卫生—中药学]