我国沈阳一起GⅡ.17型诺如病毒胃肠炎暴发的分子病原学特征研究  

The molecularly etiological study on the GⅡ.17 norovirus causing an acute gastroenteritis outbreak in Shenyang

作　　者：王冰[1] 张春青[1] 王萍[1] 张眉眉[2] 连志勇[1] 

机构地区：[1]沈阳市疾病预防控制中心,110031 [2]辽宁省疾病预防控制中心,沈阳110005

出　　处：《中华实验和临床病毒学杂志》2017年第3期208-211,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 明确沈阳地区一起学校急性胃肠炎暴发的病原和基因特征.方法 采集2015年12月沈阳地区某高校暴发疫情中的学生和食堂从业人员病例肛拭子标本共计15份,采用荧光定量RT-PCR进行诺如病毒检测,并对阳性标本进一步进行传统RT-PCR方法扩增、序列测定和系统进化分析.结果 荧光定量RT-PCR检测确定12份标本为诺如病毒阳性,系统进化分析表明,12个毒株均为GⅡ.17基因型,与引起我国2014-2015年流行的GⅡ.17新变异株KR020503株同源性为99％以上.结论 引起我国2014-2015年流行的GⅡ.17新变异株是引起此高校急性胃肠炎暴发的病原,并且GⅡ.17型诺如病毒为东北地区首次检出.Objective To define the etiology and genetics of the pathogen causing an acute gastroenteritis outbreak in a college of Shenyang.Methods A total of 15 anal swab samples were collected from students or kitchen staffs of the college where outbroke the epidemic of acute gastroenteritis in December 2015 in Shenyang,Liaoning province.Real-time PCR was performed to identify the infection of Norovirus （NoV）.The NoV genes were amplified and sequenced for those positive samples,followed to perform phylogenetic analysis.Results Of the 15 specimens,12 were NoV positive by Real-time PCR,phylogenetic analysis showed that the infected virus of the outbreak was belonged to NoV genotype GⅡ.17.The homology between the present virus and new mutated GⅡ.17 strain of KR020503 identified in 2014-2015 epidemic outbreak in China was over 99%.Conclusions The new mutated NoV GⅡ.17 caused the acute gastroenteritis outbreak in a college of Shenyang in 2015,NoV GⅡ.17 was detected firstly in Northeast China.

关 键 词：诺如病毒 急性胃肠炎 暴发 GⅡ.17型 

分 类 号：R181.3[医药卫生—流行病学] R512.5[医药卫生—公共卫生与预防医学]