深海真菌Acaromyces ingoldii FS121次级代谢产物及其细胞毒活性  

Secondary Metabolites from Deep-Sea-Derived Fungus Acaromyces ingoldii FS121 and Their Cytotoxic Activities

作　　者：高啸巍 陈玉婵[1] 孙章华[1] 刘洪新[1] 李赛妮 谭毓治[2] 章卫民[1] 

机构地区：[1]广东省微生物研究所/省部共建华南应用微生物国家重点实验室/广东省菌种保藏与应用重点实验室/广东省微生物应用新技术公共实验室,广东广州510070 [2]广东药科大学,广东广州510006

出　　处：《中药材》2017年第2期359-363,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(31272087);国家"863"计划资助项目(2012AA092104);广东省科技计划项目(2014A020217012);广州市科技计划项目(2013J4100067);广东省海洋经济创新发展区域示范专项项目(GD2012-D01-002)

摘　　要：目的:研究深海真菌Acaromyces ingoldii FS121的次级代谢产物及其细胞毒活性。方法:采用正、反相硅胶柱层析、Sephadex LH-20凝胶柱、高效液相柱色谱等各种色谱技术对菌株FS121的发酵产物进行分离纯化,采用波谱技术结合理化性质鉴定化合物结构;采用SRB法测定单体化合物体外细胞毒活性。结果:从发酵物中分离得到了9个化合物,分别鉴定为:2,4-二羟基苯甲酸(1)、胸腺嘧啶(2)、对羟基苯乙酮(3)、3-甲基-6,8-二羟基异香豆素(4)、4-甲基-5-噻唑乙醇(5)、麦角甾-4,6,8(14),22-四烯-3-酮(6)、麦角固醇(7)、(22E)-麦角甾-5,8,22-三烯-7-酮-3β-醇(8)、(3β,5α,22E)-麦角甾-6,8(14),22-三烯-3,5-二醇(9)。结论:所有化合物均为首次从Acaromyces属真菌中分离得到,化合物6对SF-268、MCF-7、NCI-H460和Hep G-2四种肿瘤细胞株具有显著的抑制活性,其IC_(50)值分别为5.3、6.5、12.2、6.1μmol/L。Objective：To study the secondary metabolites of the deep-sea-fungus Acaromyces ingoldii FS121 and their cytotoxicities.Methods：The compounds were isolated from fermentation product of the deep-sea-derived fungus Acaromyces ingoldii FS121 by silica gel,reverse phase silica gel,Sephadex LH-20,HPLC.Their structures were identified by extensive analysis of their spectroscopic data.Compounds were tested for their cytotoxicities by SRB method.Results：Nine compounds were isolated from the culture and identified as 2,4-dihydroxybenzoic acid （1）,thymine （2）,p-hydroxyacetophenone （3）,3-methyl-6,8-dihrdroxyisocoumarin （4）,4-methyl-5-thiazoleethanol （5）,ergosta-4,6,8 （14）,22-tetraen-3-one （6）,ergosterol （7）,（22E）-ergosta-5,8,22-trien-7-one-3β-ol （8）,（3β,5α,22E）-ergosta-6,8 （14）,22-triene-3,5-diol （9）.Conclusion：All compounds are isolated from Acaromyces genus for the first time.Compound 6 exhibites considerable growth-inhibitory activities in vitro against SF-268,MCF-7,NCI-H460 and HepG-2 tumor cell lines with IC50 values of 5.3,6.5,12.2,6.1 μmol/L,respectively.

关 键 词：深海真菌 Acaromyces ingoldii FS121 次级代谢产物 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]