生血宁抗红细胞衰老及对再生障碍性贫血的治疗作用  

Therapeutic Action of Shengxuening on Erythrocyte Aging and Aplastic Anemia

作　　者：李佳桓[1] 林海红[1] 杜钢军[1] 

机构地区：[1]河南大学药学院药物研究所,河南开封475004

出　　处：《中药材》2017年第2期446-451,共6页Journal of Chinese Medicinal Materials

基　　金：河南大学省部共建项目(SBGJ090704);河南省高校青年骨干教师项目(2010GGJS-025);河南省科技厅重点攻关项目(112101310308)

摘　　要：目的:观察生血宁抗红细胞衰老及对再生障碍性贫血的治疗作用。方法:体外氧化衰老和自然衰老评价生血宁的抗红细胞衰老作用,检测指标包括红细胞溶血率、Na-K-ATP酶、超氧化物歧化酶(SOD)、丙二醛(MDA)、红细胞膜渗透脆性、流动性及通透性、造血祖细胞集落生成。苯诱导的再生障碍性贫血模型观察生血宁对小鼠再生障碍性贫血的治疗作用,检测指标包括外周血红细胞(RBC)、网织红细胞(Ret)和血红蛋白(Hb)及血清红细胞生成素(EPO)、干细胞因子(SCF)、MDA和SOD。结果:生血宁能提高红细胞膜Na-K-ATP酶活性,降低MDA含量,防止红细胞的自然老化和氧化老化,显著降低红细胞膜渗透脆性及通透性,提高红细胞膜流动性。此外生血宁能刺激CFU-E、CFU-GM和CFU-F造血祖细胞集落生成。在再生障碍性贫血小鼠模型中,生血宁(400、200、100 mg/kg)呈剂量依赖性升高红细胞计数,显著提高红细胞血红蛋白含量和小鼠网织红细胞数,增加血清干细胞因子SCF、降低MDA含量。结论:生血宁有较强的抗红细胞衰老作用,对苯诱导的小鼠再生障碍性贫血有显著疗效。Objective：To study therapeutic action of Shengxuening on erythrocyte aging and aplastic anemia.Methods：The anti-aging effect of Shengxuening on erythrocytes was evaluated by erythrocyte oxidative aging and natural aging in vitro,the index included erythrocyte hemolysis rate,Na-K-ATP enzyme,SOD and MDA,red cell membrane osmotic fragility,mobility and permeability and hematopoietic progenitor cell colony formation were measured.The aplastic anemia model induced by benzene was used to observe the therapeutic action of Shengxuening on aplastic anemia in mice,the index included peripheral blood RBC,Ret and Hb,serum levels of iron,EPO,SCF,MDA and SOD were measured.Resuhs：Shengxuening could prevent natural aging and oxidative aging in erythrocytes by increasing Na-K-ATP enzyme activity and decreased the content of MDA.Shengxuening could reduce the cell membrane fragility and permeability,and increase the membrane fluidity in erythrocytes.In addition,Shengxuening significantly stimulated hematopoietic progenitor cells colony formation,such as CFU-E,CFU-GM and CFU-F.In the aplastic anemia model,Shengxuening（400,200,100 mg/kg）enhaned red blood cell count in a dose-dependent manner,and markedly increased Hb content and Ret.Shengxuening significantly increased serum SCF and decreased serum MDA content.Conclusion：Shengxuening shows a stronger anti-aging effect on erythrocytes,and has also significant therapeutic action on aplastic anemia in mice.

关 键 词：生血宁 红细胞衰老 再生障碍性贫血 造血祖细胞 

分 类 号：R285.5[医药卫生—中药学]