白藜芦醇减轻异丙肾上腺素诱导的大鼠心肌细胞肥大  

Resveratrol alleviates ISO-induced cardiomyocyte hypertrophy in rat

作　　者：王珺[1] 肖薇[1] 李波[1] 金莉[1] 于秀文[1] 廉洁[1] 李梦瑶[1] 李鑫鹏[1] 周晋宇[1] 林岩[1] 

机构地区：[1]齐齐哈尔医学院病理生理学教研室,黑龙江齐齐哈尔161006

出　　处：《基础医学与临床》2017年第9期1226-1230,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81100170);齐齐哈尔医学院科研基金(QY2016B-07;QY2016Z-14)

摘　　要：目的探讨白藜芦醇(RES)对异丙肾上腺素(ISO)诱导的大鼠心肌细胞肥大的保护作用及对心肌肥大时内质网应激相关因子GRP78和GRP94表达的影响。方法建立ISO诱导大鼠心肌细胞肥大模型,将乳鼠心肌细胞分为正常对照组、异丙肾上腺素组(加入ISO 0.3μg/m L作用48 h)、白藜芦醇干预组(RES 11.4μg/m L作用48 h)和白藜芦醇对照组。采用Leica 2Q500图像分析系统检测心肌细胞表面积和心肌肥大标志基因心房钠尿肽(ANP)表达,评价心肌细胞肥大程度;检测细胞培养液中乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量;实时定量PCR,检测ANP mRNA基因表达和Western blot分析GRP78和GRP94的蛋白表达。结果异丙肾上腺素注射液组与正常对照组比较,ISO作用心肌细胞48 h诱导心肌细胞肥大后,内质网应激相关因子GRP78和GRP94蛋白表达均增高(P<0.01,P<0.05);白藜芦醇干预组与异丙肾上腺素组比较,RES干预可以有效抑制ISO诱导的心肌细胞肥大(P<0.05);同时降低GRP78和GRP94的蛋白表达(P<0.05,P<0.01),减少细胞培养液中乳酸脱氢酶(LDH)和丙二醛(MDA)的释放(P<0.05)。结论 RES通过抑制心肌细胞内质网GRP78和GRP94表达,发挥对心肌肥大的保护作用。Objective To explore the role of resveratrol （RES） on cardiomyocyte hypertrophy of rat induced by iso- proternol （ISO） and the effect of Res on the expression of GRP78 and GRP94 in endoplasmic reticulum stress of cardiomyoeyte hypertrophy. Methods Hypertrophic model of cardiomyocytes was induced by ISO. Cardiomyocytes was divided into four group： control group, the model group, RES＋ISO group and RES group. Hypertrophy status of cardiomyocytes was determined by Leica 2Q500 image analysis system measuring the cell surface area and the gene expression of ANP. The content of LDH and MDA was measured in different groups, and the protein expressions of GRP78 and GRP94 were detected by Western blot.Results Compared with control group, ISO induced cardiomyocytes hypertrophy, endoplasmic reticulum stress related factors GRP78 and GRP94 protein expression were increased, compared with ISO group, RES intervention effectively suppressed the cardiomyocytes hyper-trophy induced by ISO, reduced the protein expression of GRP78 and GRP94, at the same time, reduced lactate dehy- drogenase （LDH） and malondialdehyde （MDA） release in cell medium. Conclusions Treatment of RES may protect cardiomyocytes hypertrophy, which is partially mediated by inhibiting the expression of ERS factors GRP78 and GRP94.

关 键 词：心肌肥大 内质网应激 白藜芦醇 GRP78 GRP94 

分 类 号：R54[医药卫生—心血管疾病]