Fat1转基因牛奶对小鼠健康和生殖能力的影响  

The Health and Reproduction of The Mice Fed by Diets Supplemented with Milk from Fat1 Transgenic Cow

作　　者：宋丽爽[1] 高广琦[1] 魏著英[1] 白春玲[1] 苏广华[1] 张立[1] 李光鹏[1] 

机构地区：[1]内蒙古大学实验动物研究中心,呼和浩特010071

出　　处：《实验动物科学》2017年第3期28-33,37,共7页Laboratory Animal Science

基　　金：国家科技转基因重大专项;高产优质转基因肉牛新品种培育(No.2014ZX08007-002)

摘　　要：目的通过饲喂试验,分析fat1转基因牛奶对小鼠行为、健康和生殖能力等的影响。方法取4周龄的B6D2F1/Vr小鼠36只,随机分成6组,每组6只,雌雄各半。分别在饲料中添加fat1转基因牛奶、克隆牛奶和普通牛奶,对上述3组鼠进行饲喂。定期对饲喂小鼠进行称质量,并统计摄食量;在饲喂14周后,每组小鼠均1∶1合笼;生育3次后,记录各组产仔量。同时,对每组小鼠进行血常规和血清生化指标分析。在整个试验期间,定期观察3组小鼠行为,并测量体质量。最后,处死小鼠,分别取各个器官进行称质量。结果与饲喂普通牛奶组相比,饲喂转基因牛奶组和克隆牛奶组的小鼠在体质量、行为、繁殖能力和大部分重要的血液生理生化指标、组织器官的质量等方面均没有显著差异。结论 fat1转基因牛奶对小鼠的健康和生殖能力没有明显不利的影响,表明fat1转基因牛奶对于动物的饮食是安全的。Objective To investigate the impact of fatl transgenic cow milk on mouse behavior, heath and reproduction. Method Thirty six B6D2F1/Vr mice at 4 weeks old were randomly divided into 6 groups with the same number of males and females in each group. The milk derived from the fatl transgenic cow, somatic cell nuclear transfer cow, and dairy cow were respectively supplemented into the diets. The milk-contained diets were respectively fed the 3 groups of mice. During feeding the behaviors such as food intake, moving, oestrus, mating were recorded and weighed regularly. After feeding female in 1：1 until produced 3 times, the offspring for 14 weeks, the mice were continuously mated by male and were counted. The blood and serum parameters and the organs were analyzed. Result Theresult showed that the growth curve , food intake, mating behaviors, reproduction, and weights of organs were with no difference among the 3 groups. Conclusion The diets supplemented with the fatl transgenic cow milk had no harmful effects on the health and reproduction of the mice, which suggest that the fatl transgenic cow milk be safe for animal diets.

关 键 词：克隆牛 转基因牛 牛奶 健康 生殖能力 血液生化 

分 类 号：Q95-33[生物学—动物学]