松叶牡丹离体快速繁殖及瓶苗微型化培育技术研究  

Techniques Onin-vitro Rapid Propagation and Miniaturization cultivation of Bottle Seedling for Lampranthustenuifolius

作　　者：黄志明[1] 沈仕泰 

机构地区：[1]莆田学院环境与生物工程学院,福建莆田351100

出　　处：《聊城大学学报（自然科学版）》2017年第2期56-59,80,共5页Journal of Liaocheng University:Natural Science Edition

基　　金：福建省教育厅科技项目(JB12177)资助

摘　　要：应用植物离体快繁技术对松叶牡丹瓶苗微型化培育技术的研究,结果表明:松叶牡丹的种子用0.1%的氯化汞灭菌5min效果最理想,成活率达到73.3%;最佳的增殖培养基配方:MS+6-BA 1.0mg·L^(-1)+IBA 0.05mg·L^(-1)+蔗糖30g·L^(-1)+琼脂9g·L^(-1);对松叶牡丹的壮苗情况来看,PP333的最佳质量浓度为0.5mg·L^(-1),芽苗在的培养基为MS+NAA 0.25mg·L^(-1)+PP3330.5mg·L^(-1)+蔗糖30g·L^(-1)+琼脂9g·L^(-1),植株矮壮,茎粗,叶片厚,茎叶红,根系发,有利瓶苗的花芽分化.Techniques about in-vitro rapid propagation for miniaturization breeding of Lampranthustenuifolius were presented.Results show that the way that utilizes 0.1% mercuric chloride liquid to sterilize seeds of Lampranthustenuifolius for 5minutes is the best,and the survival rate of these seeds can reach 73.3%.Optimum culture medium for proliferation includes MS＋6-BA 1.0mg·L^（-1）＋ IBA0.05mg·L^（-1）,sucrose 30g·L^（-1） and agar 9g·L^（-1）.Additionally,growth of strong seedling illustrates that the best mass concentration of PP333 is 0.5mg·L^（-1）.Culture medium for bud seedling contains MS＋NAA 0.25mg·L^（-1）,PP3330.5mg·L^（-1）,sucrose 30g·L^（-1）and agar 9g·L^（-1）.These plants are short but strong,and they have well developed root system,thick and red stems as well asleaves.It is beneficial to the in-vitro flower bud differentiation.

关 键 词：松叶牡丹 培养基 快繁 矮化 微型化 

分 类 号：S681.1[农业科学—观赏园艺]