多指标综合评分正交试验法优化熟地黄的炮制工艺  

Optimization of the Processing Technology of Rehmanniae Radix Praeparata by Muti-indexes Integrating Score-Orthogonal Test

作　　者：屠万倩[1] 周志敏[2] 张留记[1,2] 刘晓苗[2] 张宝[1] 崔伟峰[1] 李开言[1] 周丽[1] 

机构地区：[1]河南省中医药研究院中药研究所,郑州450004 [2]河南中医药大学药学院,郑州450008

出　　处：《中国药房》2017年第22期3121-3124,共4页China Pharmacy

基　　金：国家中医药管理局重点研究室建设项目(No.0907291);河南省重点科技攻关计划项目(No.102102310018)

摘　　要：目的:优化熟地黄的炮制工艺。方法:以梓醇、地黄苷D、毛蕊花糖苷、异毛蕊花糖苷和多糖的转移率为指标进行综合评分,以蒸制温度(压力)、蒸制时间和蒸制次数为考察因素,采用L_9(3~4)正交试验优化熟地黄的炮制工艺并进行验证试验。结果:熟地黄的最优炮制工艺为在蒸制温度125℃、蒸制压力150 kPa下蒸制2次,每次2 h;验证试验中3批样品的综合评分分别为0.698 5、0.675 5、0.701 6,各指标的RSD均小于5%(n=3)。结论:优化的炮制工艺简单、稳定、可行,可为熟地黄的工业化炮制生产提供参考。OBJECTIVE：To optimize the processing technology of rehmanniae radix praeparata. METHODS：Using transfer rates of catalpol,rehmaionoside D,acteoside,isoacteoside,polysaccharide as indexes for comprehensive score,heating temperature（pressure）,heating time and heating times as investigating factors,L9（3^4）orthogonal test was used to optimize the processing technology of rehmanniae radix praeparata,and verification test was conducted. RESULTS：The optimal processing technology of rehmanniae radix praeparata was as follow as heating temperature of 125 ℃,pressure of 150 kPa for twice,2 h every time. The comprehensive scores of 3 batches of samples were 0.698 5,0.675 5,0.701 6 in the verification test,respectively,RSDs were less than 5%（n=3）. CONCLUSIONS：Optimized processing technology is simple,stable,feasible,and can provide reference for industrial production of rehmanniae radix praeparata.

关 键 词：熟地黄 正交试验 炮制工艺 多指标 综合评分 

分 类 号：R283[医药卫生—中药学]