微生物浸出过程中胞外蛋白的作用及其机理研究进展  

The Research Progress of the Functions and Mechanism of Extracellular Proteins in Bioleaching

作　　者：余润兰[1,2] 刘阿娟[1,2] 刘亚楠[1,2] 刘学端[1,2] 邱冠周[1,2] 曾伟民[1,2] YU Run-lan LIU A-juan LIU Ya-nan LIU Xue-duan QIU Guan-zhou ZENG Wei-min(School of Minerals Processing and Bioengineering, Central South University, Changsha 410083 Key Laboratory of Biometallurgy , Ministry of Education, Central South University, Changsha 410083)

机构地区：[1]中南大学资源加工与生物工程学院,长沙410083 [2]中南大学生物冶金教育部重点实验室,长沙410083

出　　处：《生物技术通报》2017年第7期62-68,共7页Biotechnology Bulletin

基　　金：国家自然科学青年基金资助项目(31200382);国家自然科学基金面上资助项目(31470230);国家自然科学基金国际合作重大项目(51320105006);国家重点基础研究发展计划资助项目(2010CB630903);中南大学贵重仪器设备开放共享基金资助(CSUZC201603)

摘　　要：矿物-微生物-溶液多相界面是硫化矿生物浸出的关键场所,界面微生物胞外多聚物(EPS)关键组分胞外蛋白在生物膜形成、结构稳定和硫化矿溶解等方面起到关键作用。综述了生物浸出过程中EPS关键组分胞外蛋白的研究进展和胞外蛋白现有的研究方法及其对冶金微生物胞外蛋白研究的适用性,展望了浸矿微生物胞外蛋白研究的前景,旨为生物冶金领域研究EPS关键组分胞外蛋白结构及其作用机理提供重要的理论和方法支撑。Mineral-microbe-solute multi phase interface is the key space for bioleaching of sulfide minerals. Microbial proteins at theinterface as the key components of extracellular polymeric substances（EPS）play a key role in the biofilm formation,structure maintainanceand sulfide mineral dissolution. This article reviews the developments,existing methods and their applicability for studying the extracellularproteins in EPS of metallurgical microorganisms during bioleaching,and prospects a vista of future research on the extracellular proteinproduced by bioleaching microorganisms. These will offer important theoretical basis for studying the structure and functional mechanisms ofextracellular proteins in the bioleaching process.

关 键 词：生物浸出 胞外多聚物(EPS) 胞外蛋白 激光共聚焦显微镜(CLSM) 

分 类 号：Q936[生物学—微生物学] TF18[冶金工程—冶金物理化学]