冬枣轮纹病菌拮抗放线菌的筛选、鉴定及其抑制效果  

Screening,Identification,and Inhibitory Effect of Antagonistic Actinomycetes Against Macrophoma kuwatsukai Causing Winter Jujube Ring Grain Disease

作　　者：范延辉[1] 王君[2] FAN Yan-hui WANG Jun(Research Center for Eco-Environmental Sciences Yellow River Delta, Shandong Provincial Key Laboratory of Eco-Environmental Science for Yellow River Delta, Binzhou 256603 School of Bioengineering, Binzhou University, Shandong Provincial Engineering and Technology Research Center for Wild Plant Resources Development and Application of Yellow River Delta, Binzhou 256603 i)

机构地区：[1]黄河三角洲生态环境研究中心山东省黄河三角洲生态环境重点实验室,滨州256603 [2]滨州学院生物工程学院山东省黄河三角洲野生植物资源开发利用工程技术研究中心,滨州256603

出　　处：《生物技术通报》2017年第7期114-119,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31000059);山东省高等学校科技计划项目(J10LC55);国家级大学生创新创业训练计划项目(201410449034)

摘　　要：旨在筛选出能高效抑制冬枣轮纹病病原真菌的放线菌,为冬枣轮纹病的生物防治提供理论依据。通过纯培养法从黄河三角洲贝壳堤岛贝沙沉积物中分离获得198株放线菌,对抑菌作用最强的菌株BK作了进一步研究。通过形态特征、生理生化特征和16S rRNA序列分析对菌株BK进行了鉴定,并研究了其对冬枣轮纹病菌抑制效果。结果表明,菌株BK对冬枣轮纹病菌丝生长抑制率达72.30%。菌株BK发酵液对离体冬枣果实轮纹病的防效为73.72%,与50%多菌灵的防效相差不显著。经鉴定菌株BK为绛红北里孢菌是一株较为理想的拮抗菌,在冬枣轮纹病生物防治方面有较好的应用前景。The objective is to select actinomycete which has strong antagonistic ability against winter jujube ring grain disease. Total 198 actinomycetes were isolated by culture-dependent method from the shell sediment in the Seashell Islands of Yellow River Delta,and the strainBK with the strongest antagonistic ability was further studied. Strain BK was identified based on its morphology,physio-biochemical charactersand 16 S rRNA sequence analysis,and its ability against winter jujube ring grain disease was also studied,providing a theoretical basis inbiological control of winter jujube ring grain disease. The results showed that BK significantly inhibited the mycelium growth of Macrophoma kuwatsukai,with inhibitory rate of 72.3%. The control effect of the culture filtrate against winter jujube ring grain disease was 73.7%,whichhad no significant difference from that by the 50% carbendazim. Strain BK was identified as Streptomyces purpureus. To sum up,strain BK is anideal antagonistic one,and presents a potential application for the biological control of winter jujube ring grain disease.

关 键 词：黄河三角洲贝壳堤岛 拮抗放线菌 冬枣 轮纹病 抑制效果 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]