铁蓄积对骨内H型血管影响的实验研究  

Experimental research on the influence of iron accumulation on type H vessel in bone

作　　者：王亮[1] 韩小娟[1] 赵国阳[3] 单宇 王爱飞[1] 刘志鹏[1] 陈斌[1] 俞晨[1] 徐又佳[1] 

机构地区：[1]苏州大学附属第二医院骨科,215004 [2]吴江区第一人民医院骨科,苏州215200 [3]江苏大学附属医院骨科,镇江212001

出　　处：《中华骨科杂志》2017年第14期864-870,共7页Chinese Journal of Orthopaedics

基　　金：国家自然科学基金（81572179）;江苏省重点研发（社会发展）项目（BE2016720）;苏州市科技计划项L1（SYS201637）;苏州市吴江区第一人民医院立项课题（201615）;苏州大学青年教师科学基金项目（SDY2011A28）

摘　　要：目的探讨铁蓄积导致骨量下降的过程中骨内H型血管的变化和相关意义。方法选取10只雄性8周龄野生型C57BL／6J小鼠，随机分为对照组及铁蓄积组，每组5只。对铁蓄积组腹腔注射0．1g／kg右旋糖酐铁，1次／周，连续注射8周，对照组注射等量生理盐水。取股骨、胫骨标本分别进行骨组织Micro—CT扫描、骨组织H型血管免疫荧光染色。收集两组小鼠肝脏组织通过原子吸收法检测铁含量变化，并对各组间的结果进行统计学分析。结果铁蓄积小鼠肝铁含量较对照组显著升高，模型制作成功。收集两组小鼠胫骨进行免疫荧光实验：铁蓄积组小鼠胫骨干骺端骨内H型血管面积为111．24％±1．76％，对照组H型血管面积为30．69％±2．78％，两组间血管面积差异有统计学意义（P〈0．005）。收集两组小鼠股骨行Micro—CT扫描：铁蓄积组骨密度（bone mineral density，BMD），体积分数bone volume／tissue volume，BV／TV），骨小梁厚度（trabecular thickness，Tb．Th），骨小梁数目（trabeeular number，rb．N），骨小梁间隙（trabecular separation，Tb．Sp）分别为（0．19±0．013）g／cm^3，11．92％±1．199％，（35．66±2．684）μm，（2．36±0．429）个／mm，（284．41±23．197）μm；对照组BMD，BV／TV，Tb．Th，Tb．N，Tb．Sp为（0．37±0．023）g／cm^3，35．76％±1．336％，（62．05±2．238）μm，（5．68±1．039）个／mm，（163．23±13．203）μm，两组问差异均有统计学意义（P〈0．05）。结论铁蓄积导致了骨密度下降，同时骨内H型血管的形成显著受到抑制，这对了解铁蓄积导致骨质疏松症的作用机理有新的实验价值。Objective To explore the changes of type H vessel during the low bone mineral density caused by iron accu- mulation and discuss its clinical meaning. Methods Ten 8-week old male C57BL/6J mice were used for experiments, and ran- domly divided into two groups： control group and iron group, and 5 mice in each group. In the iron group, 0.1 g/kg of iron dextran was injected intraperitoneally once a week for 8 weeks. The control group was injected with the same amount of saline. The femoral and tibial specimens were examined by microscopic CT scan and bone tissue type H vessel immunohistochemical staining. Liver tissue from the two groups were collected for the content of iron by atomic absorption spectroscopy. All experimental data were ana- lyzed with t-test. Results The content of hepatic iron in mice was significantly higher than that in the control group, which indicating that the model was successfully established. The tibia specimens were collected for immunostaining. The vascular area of type H at metaphyseal regions is 11.24%±1.76% in iron group and 30.69%±2.78% in control group, respectively. There is significant difference between the two groups （P〈0.005）. The femur specimens were collected for Micro-CT scan, the value of bone mineral density （BMD）, bone volume/tissue volume （BV/TV）, trabecular thickness （Tb. Th）, trabecular number （Tb. N） and trabecular separation （Tb. Sp） was （0.19±0.013） g/cm^3, 11.92%± 1.199%, （35.66±2.684）/xm, （2.36±0.429） Imm and （284.41 ±23.197） μm in iron group and （0.37±0.023） g/cm3, 35.76%1.336%, （62.05±2.238）/xm, （5.68± 1.039）/ram and （163.23± 13.203） μm in control group, respectively. The differences between the two groups were statistically significant （P〈0.05）. Conclusion Iron accumulation can lead to low BMD and suppress type H vessel formation in bone, which might provide a new experimental value for mechanism research on osteoporosis caused by iron accumulation.

关 键 词：动物实验 骨密度 骨质疏松 

分 类 号：R580[医药卫生—内分泌]