半夏泻心汤和鸟嘌呤核苷对APPswe/PS1dE9双转基因小鼠学习记忆的影响  

Influences of Banxia Xiexin Tang and guanosine on learning and memory in APPswe/PS1dE9 double transgenic mice

作　　者：盛宁[1] 何瑛琨[1,2] 冯慧利[1] 董云芳[1] 樊惠[1,3] 党惠子[1,4] 任映[1] 杨金铎[1] 王蓬文[1] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部和北京市重点实验室,北京100700 [2]河北省人民医院 [3]北京朝阳区中西医结合急诊抢救中心 [4]北京市回民医院

出　　处：《北京中医药大学学报》2017年第7期558-563,共6页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上资助项目(No.81573927);北京市教育委员会科学研究与研究生培养共建资助项目(No.2016)~~

摘　　要：目的采用跳台和Morris水迷宫检测半夏泻心汤和鸟嘌呤核苷对6月龄APPswe/PS1d E9双转基因小鼠学习记忆的影响。方法将APPswe/PS1d E9双转基因小鼠随机分为模型组5%羧甲基纤维素钠(CMC)灌胃、西药对照组盐酸多奈哌齐0.92 mg/(kg·d)灌胃、半夏泻心汤组650 mg/(kg·d)灌胃、中药对照组姜黄素200 mg/(kg·d)灌胃、鸟嘌呤核苷组0.5%CMC灌胃,除鸟嘌呤核苷组腹腔注射鸟嘌呤核苷16 mg/(kg·d),其他各组腹腔注射等体积的生理盐水,每组15只。正常组0.5%CMC灌胃和等体积的生理盐水腹腔注射为同窝野生型C57BL/6J小鼠。于3月龄时开始对各组小鼠分别实施对应的干预,给药3个月后,应用跳台和Morris水迷宫对小鼠进行行为学检测。结果跳台实验结果显示,模型组小鼠比正常组潜伏期时间明显缩短(P<0.01);与模型组比,各干预组小鼠潜伏期时间均显著增加(P<0.01)。Morris水迷宫结果显示,随着天数的增加,各组小鼠逃避潜伏期的时间缩短,游泳距离也呈递减趋势,中间有小幅波动。Morris水迷宫结果显示,空间探索实验即撤台实验中,模型组小鼠目标象限停留时间显著少于正常组(P<0.01),西药对照组、中药对照组以及半夏泻心汤组小鼠的目标象限停留时间均比模型组延长(P<0.05)。结论半夏泻心汤对APPswe/PS1d E9双转基因小鼠的早期学习记忆障碍具有改善作用,鸟嘌呤核苷可提高小鼠的记忆保持能力。Objective To detect the influences of Decoction randomly ） and d） by divided Banxia Xiexin Tang （Pinellia Heart-Draining guanosine on learning and memory in APPswe/PS1dE9 double transgenic mice （6 applying step-down test and Morris water maze （MWM）. Methods The mice into model group （given 0.5% CMC intragastrically）, Western drug control were group [ WD group, given donepezil in 0.92 mg/（ kg · d） intragastrically], Banxia Xiexin Tang group [ BXT group, given Banxia Xiexin Tang in 650 mg/（ kg· d） intragastrically], Chinese medicinal control group [CM group, given curcumin in 200 mg/（kg · d）] and guanosine group （given 0. 5% CMC intragastrically, each n = 15）. The guanosine group was given intraperitoneal injection of guanosine [ 16 mg/（kg · d）] , and other groups were given intraperitoneal injection of isopyknic normal saline （NS）. The brood wide type C57BL/6J mice （ n = 15 ） were set into normal group and given 0. 5% CMC intragastrically and isopyknic NS. All groups were given corresponding interventions when mice were 3 months old. The ethological detection was conducted by using step-down test and MWM in al groups after 3 m. Results The results of step-down test showed that latency was significantly shorter in model group than that in normal group （P 〈 0.01 ）, and was significantly longer in all medicinal intervention groups than that in model group （P 〈0.01 ）. The results of MWM showed that escape latency was shortened, swimming distance showed decline trend with slight fluctuation in all groups along with days increasing. The results of MWM showed that in spatial probe test, the time of targeting quadrant was significantly shorter in model group than that in normal group （ P 〈 0.01 ）, and was longer in WD group, CM group and BXT group than that in model group （ P 〈 0. 05 ）. Conclusion Banxia Xiexin Tang has an ameliorative effect on early learning and memory disorder, and guanosine can improve the ability of memory retaining in APPswe/PS

关 键 词：鸟嘌呤核苷 半夏泻心汤 阿尔茨海默病 行为学 小鼠 

分 类 号：R285.5[医药卫生—中药学]