贵州地方山羊ERα多态性及组织差异表达  

Polymorphism and Tissue Differential Expression of ERα gene in Guizhou Goats(Capra hicus)

作　　者：李鹏程[1] 孙振梅[1] 许厚强[1] 陈祥[1] 

机构地区：[1]贵州大学动物科学学院/高原山地遗传育种与繁殖省部共建教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室,贵阳550025

出　　处：《农业生物技术学报》2017年第8期1300-1306,共7页Journal of Agricultural Biotechnology

基　　金：贵州省科技重大专项[黔科合重大专项字2013(6008)];贵州大学研究生创新基金(研农2016024)

摘　　要：雌激素受体α(estrogen receptor,ERα)基因在动物繁殖过程中起着重要作用,已经被认为是影响猪(Sus scrofa)产仔数的主效基因,但在山羊(Capra hicus)方面研究较少。为了揭示雌激素受体基因对贵州本地山羊繁殖性状的影响,本研究采用直接测序技术检测了该基因8个外显子的SNP,并使用qRT-PCR检测ERα基因在黔北麻羊的下丘脑,垂体,性腺轴中不同表达量。结果表明,ERα基因在黔北麻羊和贵州黑山羊第8外显子中均存在C127T突变,且属于错义突变,致使该基因mRNA二级结构以及编码蛋白结构的改变。在第8外显子的C127T位点发现CC、CT和TT 3种基因型,其中,在黔北麻羊群体中CC基因型个体的产羔数与TT和CT基因型间达差异显著水平(P<0.05),在贵州黑山羊群体中未达到显著差异。qRT-PCR检测结果显示,单羔组和多羔组黔北麻羊的5个组织中ERα基因均有表达,且卵巢均最高。进一步研究发现,单羔组和多羔组黔北麻羊卵巢组织中的ERαmRNA表达量差异显著(P<0.05),而在其他组织间均未达到差异显著(P>0.05)。结果显示,ERα中的C127T突变可能影响贵州地方山羊的产羔数,为培育贵州地方山羊高繁殖力品系提供了理论依据。Estrogen receptorα （ERα） gene plays an important role in the process of animal breeding. It has been considered as the main gene affecting the litter size of swine, but it is hardly studied in goat （Ovis linnaeus）. In order to reveal the effects of ERa gene on reproductive traits of Guizhou local goat, the polymorphism of ERα gene 8 exons was detected by direct sequencing and the ERa mRNA expression in Qianbei Ma goat hypothalamus, pituitary and gonadal axis was detected by qRT-PCR. The results showed that C 127T existed in the eighth exon of ERa gene of Qianbei Ma goat and Guizhou black goat. The C 127T was a missense mutation and caused the change of mRNA secondary structure and encoding protein of ERα gene. CC, CT and TT genotypes were detected at C127T locus of the eighth exon of ERα gene. The Qianbei Ma goat with CC achieved significant difference level compared to CT and CC genotype. But the Guizhou Black goat did not reach significant differences, qRT-PCR results showed that the ERα gene in 2 groups （single lamb group and more lamb group） were expressed in 5 tissues of the Qianbei Ma goat. The highest in ovaries, the lowest in the fallopian tube, and the mRNA expression in the two groups of North Guizhou sheep tissues did not reach significant levels （P〉0.05）. These results indicated that C127T mutation of ERa gene might have an effect on reproduction traits of local goats in Guizhou province. This study provided the theoretical basis for fostering the high fecundity lines of local goats in Guizhou.

关 键 词：黔北麻羊 贵州黑山羊 单核苷酸多态性 QRT-PCR 雌激素受体α(ERα) 

分 类 号：S827[农业科学—畜牧学]