霍恩氏法与序贯法在急性经口毒性试验中应用研究  

Application of Horn's and Dixon-Mood in Acute Oral Toxicity Test of Pesticides

作　　者：于春[1] 李军[2] 岑延利[2] 王祺[2] 秦旭[1] YU Chun LI Jun CEN Yan-li et al(School of Public Health, Guizhou Medical University, Guiyang, Guizhou 550004 Key Laboratory of Ministry of Education by Environmental Pollution and Disease Control Laboratory, Guiyang, Guizhou 550004)

机构地区：[1]贵州医科大学公共卫生学院中心实验室,贵州贵阳550004 [2]环境污染与疾病监控教育部重点实验室,贵州贵阳550004

出　　处：《安徽农业科学》2017年第21期7-8,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]探讨序贯法作为农药急性毒性替代方法的可靠性、可操作性以及该方法的优缺点。[方法]采用序贯法与霍恩氏法分别测定6种农药制剂的急性经口半数致死剂量(LD50)和95%可信区间,比较2种方法获得的急性毒性分级和试验所用动物数量。[结果]序贯法测得的LD50与霍恩氏法相近,毒性分级与霍恩氏法均一致;但序贯法试验使用动物数量减少了52.3%。[结论]序贯法测定农药急性经口LD50具有明显优势,用于农药急性经口毒性检测具有广阔前景。[ Objective ] The aim was to explore Dixon-Mood as pesticide acute toxicity alternative methods of reliability and operability and the advantages and disadvantages of this method. [ Method] The median lethal dose （LD50） and 95% confidence intervals of 6 pesticides were detected by Dixon-Mood and Horn＇ s, and the two acute toxicity classifications and the animal consumption of the two methods were compared. [ Result] The LD50 and 95% confidence intervals of the Dixon-Mood were similar with those of the Horn＇ s, and the animals could be economized by 52.3% with the Dixon-Mood compared with those of the Horn＇ s. [ Conclusion] The Dixon-Mood determination of pesticide acute tramsotal LD50 has obvious advantages, and has wide prospect in the pesticide acute tramsotal toxicity testing.

关 键 词：序贯法 霍恩氏法 半数致死剂量(LD50) 急性经口毒性试验 

分 类 号：R99[医药卫生—毒理学]