酪酸梭菌对食物过敏小鼠肠道屏障功能的影响  

Effects of clostridium butyricum on intestinal barrier function in food allergic mice

作　　者：李旌[1] 黄煌[1] 梅璐[1] 于泳[1] 刘思濛 丁一芮 白利梅[1] 蒋杰[1] 郑鹏远[1] 

机构地区：[1]郑州大学第五附属医院消化科,郑州450052

出　　处：《重庆医学》2017年第22期3028-3032,共5页Chongqing medicine

基　　金：国家自然科学基金资助项目(81370494);国家973计划前期研究专项(2011CB512006);河南省科技创新杰出人才基金(134200510022);郑州市创新型科技人才队伍建设工程基金(131PLJRC656)

摘　　要：目的探讨产丁酸的益生菌酪酸梭菌通过调节肠上皮细胞双孔钾通道Trek1的表达对过敏小鼠肠道屏障功能的影响。方法建立小鼠食物过敏模型,ELISA法、流式细胞仪检测相关指标验证造模效果,尤斯室检测小肠组织通透性变化,Western blot及免疫荧光法检测Na6ve组、Saline组、SIT组、SIT/SB组、SB组、SIT/CB组、CB组、SIT/CB/Spadin组小鼠空肠组织中Trek1的表达;Transwell系统中使用T84细胞建立单层上皮细胞层,分别暴露于过敏介质,qRT-PCR和Western blot检测Trek1mRNA和蛋白的表达;分别使用生理盐水、SIT、SIT/SB、SB、SIT/CB、CB、SIT/CB/Spadin不同疗法对过敏小鼠进行治疗,了解小鼠肠道Trek1的表达情况、肠道屏障功能及过敏反应指标的变化情况。结果相对于对照组,食物过敏小鼠小肠Trek1蛋白表达水平显著下降,肠黏膜通透性显著升高,差异有统计学意义(P<0.05);T84细胞暴露于过敏介质后,Trek1mRNA和蛋白的表达显著下降(P<0.05),但预先加入p38抑制剂可以拮抗这种改变;SIT治疗、酪酸梭菌和丁酸钠单独应用均可以提高食物过敏小鼠肠道Trek1的表达,减轻过敏反应(P<0.05),但SIT与酪酸梭菌或丁酸钠的联合应用效果更加显著(与SIT组相比P<0.05),并且可以显著降低小肠黏膜通透性,改善肠道屏障功能(P<0.05)。结论丁酸钠或者产丁酸的益生菌酪酸梭菌可以通过提高Trek1的表达来恢复过敏小鼠的肠道屏障功能,减轻过敏反应,并且加强SIT治疗的效果。Objective To investigate that butyrate producing probiotic clostridium butyricum improves the intestinal epitheli al barrier function in food allergic mice by regulating TWIK-related potassium channel-1 （Trekl） expression in intestinal epithelial cells. Methods An intestinal allergy mouse model was created, then the model construction effect was verified by detecting the re- lated indicators by ELISA,flow cytometer. The change of small intestinal tissue permeability was detected by the Ussing chambers. The Trekl expressions in mouse jejunum tissue in control group and allergy group were detected by Western blot and immunofluo rescent method;in the Transwell system,T84 cells were used to establish epithelial cellular monolayer for exposing to the allergic mediators,the Trekl mRNA and protein expression were detected by qRT-PCR and Western blot. Then the allergic mice were grouped and treated by different methods including normal saline, SIT, SIT/SB, SB, SIT/CB, CB, SIT/CB/Spadin, the expression of mice intestinal Trekl,intestinal barrier function and allergic reaction indicators were detected. Results Compared with the control group, the small intestinal Trekl protein and tissue expression level in the food allergic mice were significantly decreased, the intestinal mucosal permeability was significantly increased, the differences were statistically significant（P〈0. 05） ;after T84 cells exposing to the allergic mediators,Trekl mRNA and protein expression were significantly decreased（P〈0.05）, but adding p38 inhibitor in advance could antagonize this change the single use of SIT, clostridium butyricum and sodium butyrate could increase the intestinal Trek1 expression in food allergic mice,and alleviated the allergic reaction（P〈0.05） ,whereas the combined use of SIT and dos tridium butyrieum or sodium butyrate had more significant effect（compared with the SIT group,P〈0.05） ,moreover could significantly decrease small intestinal mucosal permeability and improved the intestinal barrier functi

关 键 词：食物过敏 Trekl 肠道屏障功能 益生菌 

分 类 号：R593.1[医药卫生—内科学]