一起聚集性腺病毒暴发疫情的调查及病原学分析  

Investigation and etiological analysis of an aggregated adenovirus outbreak

作　　者：王慎骄[1] 龚利强[2] 陈立凌[3] 许可[1] 付建光[1] 秦圆方[1] 邓斐[1] 余慧燕[1] 霍翔[1] 鲍昌俊[1] 

机构地区：[1]江苏省疾病预防控制中心,南京210009 [2]江苏省常熟市疾病预防控制中心 [3]江苏省苏州市疾病预防控制中心

出　　处：《江苏预防医学》2017年第4期407-410,共4页Jiangsu Journal of Preventive Medicine

基　　金：基金项目:江苏省重大新发传染病综合防控科技示范工程(BE2015714);江苏省卫生和计划生育委员会"科教强卫"工程医学重点人才(ZDRCA2016032)

摘　　要：目的对一起流感样暴发疫情进行流行病学调查和病原学检测,对毒株进行分子流行病学分析,为呼吸道传染病暴发疫情的处置提供依据。方法对暴发疫情进行流行病学调查,收集病例咽拭子样本,采用RespiFinder 2SMART多病原检测试剂盒进行熔解曲线分析,筛查致病原,并以qPCR法确认。用Hep-2细胞分离病毒,提取毒株核酸进行PCR扩增并进行序列分析,NCBI核酸数据库进行序列比对,确定型别。结果疫情累计发病17例,仅涉及一个班级,班级罹患率43.6%,男女比1.8∶1,疫情持续11d。主要症状为发热(17例,100.0%)、咳嗽(16例,94.1%)等。采集10例病例咽拭子,5份以多病原检测试剂盒进行熔解曲线分析,检出2例为腺病毒(ADV)核酸阳性,其余5份经qPCR检测,3份为ADV核酸阳性。以Hep-2细胞分离5份ADV核酸阳性标本,成功分离4株毒株,对其进行DNA测序,NCBI核酸数据库进行Blast序列比对,结果显示其六邻体保守区核酸与ADV3型核酸同源性为97%。结论该暴发是一起由ADV3型引起的暴发疫情。Objective To carry out epidemiological investigation and etiological analysis on an influenza-like outbreak ;to isolate virus strains and analyze their molecular characteristics ;to provide experimental evidences for prevention and control of respiratory infectious disease outbreaks. Methods Epidemiological investigation was carried out for the outbreak. Throat swab samples were collected and analyzed by RespiFinder 2SMART multi pathogen analysis kit to identify pathogen by melting curve analysis, which was confirmed by qPCR method. Hep-2 cell line was used for virus isolation. The nucleic acid of virus was ex- tracted and amplified for hexon gene by PCR, followed by sequence analysis and Blast in NCBI nucleotide database for to confirm the subtype. Results A total of 17 cases occurred in this outbreak, which all occurred in only one class, resulting class incidence of 43.6% . The ratio of male patients to female patients was 1.8 to 1. The main symptoms were fever （17 cases, 100.0K） and cough （16 cases, 94.1%）, etc. A total of 5 throat swabs of 10 collected samples were analyzed by multiple pathogens analysis kit, among which 2 were detected positive for adenovirus （ADV） nucleic acid; the other 5 samples were an- alyzed by qPCR, among which 3 were positive for ADV nucleic acid. Hep-2 cells were used to isolate 5 ADV nucleic acid posi- tive samples, a total of 4 isolated stains were obtained. DNA sequencing and Blast analysis showed the DNA of hexon gene of isolated viruses had 970~ homology with that of ADV3. Conclusion The outbreak was caused by ADV3.

关 键 词：腺病毒3型 熔解曲线 流行病学调查 病原学分析 序列比对 暴发疫情 

分 类 号：R511.8[医药卫生—内科学]