小盐芥TsPIP1;1与TsTIP1;1基因增强转基因水稻耐盐性  

Thellungiella salsuginea Ts PIP1;1 and Ts TIP1;1 enhanced the salt tolerance of transgenic rice

作　　者：李伟[1] 韩娇[2] 黄升财 何蕊[1,3] 王冰[1] 程宪国[1] LI Weil HAN Jiao HUANG Sheng-cai HE Rui WANG Bing CHENG Xian-guo(Key Laboratory of Plant Nutrition and Fertilizer, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China College of Life Scienee, Shanxi Normal University, Linfen, Shanxi 041000, China College of Land and Environmental Sciences, Shenyang Agricultural University, Shenyang 110866, China)

机构地区：[1]中国农业科学院农业资源与农业区划研究所,北京100081 [2]山西师范大学生命科学学院,山西临汾041000 [3]沈阳农业大学土地与环境学院,辽宁沈阳110866

出　　处：《植物营养与肥料学报》2017年第4期957-963,共7页Journal of Plant Nutrition and Fertilizers

基　　金：国家重点研发项目(2016YFC0501203);973项目(2015CB150800)资助

摘　　要：【目的】为更好地了解植物水通道蛋白盐胁迫下的调节作用,对小盐芥质膜内在蛋白Ts PIP1;1及液泡膜内在蛋白Ts TIP1;1在转基因水稻中的盐胁迫生理响应机制进行探究,旨在为水通道蛋白在耐盐作物分子改良育种中的应用提供理论支撑。【方法】以野生型(WT)与T3代转Ts PIP1;1及Ts TIP1;1基因水稻为材料,进行了水培试验,并设置了0、100、200 mmol/L Na Cl处理。处理一周后,分别测定水稻的光合参数、株高、生物量、相对含水量、失水率及钾、钠含量。【结果】在盐胁迫处理下,与野生型相比,转基因水稻的生物量和含水量明显增加,渗透势和失水率显著降低。转Ts PIP1;1及Ts TIP1;1基因水稻根部及地上部的Na^+含量都显著降低,K^+在转基因株系中的累积显著高于野生型,降低了体内Na^+/K^+比,并且能够保持更强的净光合速率、气孔导度、蒸腾速率及水分利用效率。在200 mmol/L Na Cl处理下,与野生型相比,Ts TIP-5、Ts TIP-7及Ts PIP-19的株高分别高出8.2%、11.6%、4.9%;单株干重分别高出17.9%、23.9%、16.9%;地上部Na^+/K^+比分别降低24.3%、24.4%、24.8%;根部Na^+/K^+比分别降低29.6%、27.5%、32.4%;渗透势分别显著降低了18.3%、19.4%、30.3%;相对含水量分别增加了5.8%、5.5%、5.4%;净光合速率分别增加了50.4%、78.5%、56.2%。【结论】Ts PIP1;1及Ts TIP1;1增强了转基因水稻的光合呼吸作用,通过降低植物体内Na^+/K^+比,参与植物细胞的渗透调节,提高了细胞持水能力,促进转基因水稻的生长发育,增强了水稻的耐盐性。[ Objectives ] To better understand the regulatory roles of aquaporins in plants in response to salt stress and provide a theoretical support for the application of aquaporin genes in molecular breeding of salt tolerant crop, two aquaporins of Thellungiella salsuginea, plasma membrane intrinsic protein TsPIP1;1 and tonoplast intrinsic protein TsTIP1;1, were investigated by profiling the physiological responses in the transgenic rice under salt stress. [ Methods ] Wild type （WT） and T3 generations of the transgenic rice carrying the TsPIP1;1 and TsTIP1;1 were subjected to hydroponic solution with 0, 100 and 200 mmol/L NaC1 in greenhouse, respectively. After one-week＇s salt treatments, some important physiological parameters such as the photosynthetic rates, plant height, biomass, relative water contents, water loss rates and the contents of Na＋ and K＋ were determined. [ Results ] Under 200 mmol/L NaC1 treatment, compared with the wild type, thetransgenic rice exhibited higher biomass and relative water contents, and lower water loss rates and osmotic potentials, and demonstrated lower contents ofNa~ and higher contents of K~ in roots and shoots and thus leading to lower Na~/K＋ ratios. The transgenic rice maintained stronger net photosynthetic rate, stomatal conductance, transpiration rate and water use efficiency. Compared with the wild type, in 200 mmol/L NaC1 treatment, the shoot heights in the transgenic lines TsTIP-5, TsTIP-7 and TsPIP-19 were respectively increased by 8.2%, 11.6% and 4.9%, and plant dry weights were increased by 17.9%, 23.9% and 16.9%, while the Nat/K＋ ratios of shoots were reduced by 24.3%, 24.4% and 24.8%, and the NaVK＋ ratios of roots were decreased by 29.6%, 27.5% and 32.4%. The osmotic potentials were significantly depressed by 18.3%, 19.4% and 30.3%, the relative water contents were increased by 5.8%, 5.5% and 5.4%, and the net photosynthetic rates were increased by 50.4%, 78.8% and 56.2%. [ Conclusions ] The transgenic rice of carrying the TsPIP1;1 and TsTIP1;1 genes ha

关 键 词：转基因水稻 盐胁迫 水通道蛋白 渗透调节 

分 类 号：S511[农业科学—作物学]