对照提取物法测定补骨脂饮片中7种成分含量  

Content Determination of 7 Compounds of Psoraleae Fructus by Reference Extract Method

作　　者：朱星宇[1] 赵根华 高倩倩[1] 王恒[1] 刘玲[1] 陈志鹏[1] 吴丽[1] 李伟东[1] ZHU Xing-yu ZHAO Gen-hua GAO Qian-qian WANG Heng CHEN Zhi-peng WU Li LI Wei-dong(Department of Pharmacy, Nanjing University of Chinese Medicine, Jiangsu Key Laboratory of Chinese Medicine Processing, Nanfing 210023, China)

机构地区：[1]南京中医药大学药学院江苏省中药炮制重点实验室,南京210023

出　　处：《中国实验方剂学杂志》2017年第15期85-91,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：浙江省重中之重一级学科--中药学学科科研开放基金资助项目;江苏省高校自然科学基金重点项目(11KJA360001);江苏省高校"青蓝工程"资助项目(2014)

摘　　要：目的:为了提高补骨脂饮片的质量标准,解决部分对照品稀缺问题,建立以补骨脂对照提取物为对照的补骨脂饮片中7种成分含量测定的方法。方法:采用高效液相色谱法。Thermo-endcapped C18色谱柱(4.6 mm×250 mm,5μm),流动相水-乙腈-甲醇梯度洗脱,柱温30℃,检测波长254 nm,进样量10μL。自制了补骨脂对照提取物并标定了其中补骨脂素、异补骨脂素、补骨脂甲素、补骨脂定、补骨脂乙素、补骨脂二氢黄酮甲醚、补骨脂酚7种成分的含量,并用补骨脂对照提取物为对照测定了12批补骨脂饮片中7种成分的含量,与对照品测定法的试验结果进行比较。结果:补骨脂饮片中补骨脂素、异补骨脂素、补骨脂甲素、补骨脂定、补骨脂乙素、补骨脂二氢黄酮甲醚、补骨脂酚7种成分均能达到基线分离、线性关系良好,平均回收率分别为97.4%,98.8%,99.6%,102%,99.6%,98.0%,103%,RSD均≤2.8%。该方法的精密度、稳定性、重复性良好,并且测定结果与对照品测定法所得结果无显著性差异,2种测定方法所得数据的RSD<3%。结论:补骨脂对照提取物法可用于补骨脂饮片中7种成分的含量测定,能有效地对补骨脂饮片进行质量控制。Objective：To improve the quality standard of Psoraleae Fructus,solve the problem of lacking relevant reference substance,and establish a content determination method for 7 compounds in Psoraleae Fructus decoration piece based on the standard reference extract.Method：HPLC was performed on Thermo-C18 endcapped column（4.6 mm×250 mm,5 μm）,with water-methanol-acetonitrile system as the mobile phase for gradient elution.The column temperature was 30 ℃;the injection volume was 10 μL,and the detection wavelength was set at 254 nm.Self-prepared reference extracts of Psoraleae Fructus was used for determining the contents of psorale,isopsorale,psoralidin,corylifolinin,bavachinin and bakuchiol;then the contents of the 7 components in 12 batches of Psoraleae Fructus decoration piece were determined and compared with the experimental results obtained with the reference substance determination method.Result：The seven markers including psorale,isopsorale,psoralidin,corylifolinin,bavachinin and bakuchiol,reached baseline separation and showed good linearity.Their average recovery rate was 97.4%,98.8%,99.6%,102%,99.6%,98.0%,103% and RSDs of seven compounds were all no more than 2.8%.The reference extraction determination method showed high precision,stability and reproducibility,and there was no significant difference with the results of reference substance determination method.Conclusion：The reference extraction determination method was simple,and can be used for the contents determination of 7 compounds in Psoraleae Fructus decoration piece.

关 键 词：补骨脂 对照提取物法 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]