木瓜苦桑片对Ⅱ型糖尿病大鼠的影响  

Effect of Mugua Kusang Tablets on Type 2 Diabetes Rats

作　　者：高玲[1] 许立拔[1] 王巍[1] 林彩霞[1] 杨沛霖[1] 巫玲玲[1] 庞晓军[1] 蒋伟哲[1] GAO Ling XU Li-ba WANG Wei LIN Cai-xia YANG Pei-lin WU Ling-ling PANG Xiao-jun JIANG Wei-zhe(Pharmaceutical College, Guangxi Medical University, Nanning 530021, China)

机构地区：[1]广西医科大学药学院,南宁530021

出　　处：《中国实验方剂学杂志》2017年第15期166-172,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西科技计划项目(桂科AB16380187)

摘　　要：目的:研究木瓜苦桑片对高脂高糖联合低剂量链脲佐菌素诱导的Ⅱ型糖尿病大鼠血糖、血脂、肝肾功能和胰腺组织的影响。方法:雄性SD大鼠给予高脂饲料喂养4周后,链脲佐菌素(45 mg·kg^(-1))给药1次,建立糖尿病大鼠模型,将造模成功的大鼠随机分为模型组,二甲双胍组(285 mg·kg^(-1)),木瓜苦桑片高、中、低剂量组(2 600,1 300,650 mg·kg^(-1)),另设正常组,每组10只,每天灌胃给药1次,连续4周,记录每只大鼠24 h的尿量、饮水量、摄食量,分别测定血糖值(FBG),胰岛素(INS),过氧化氢酶(CAT),超氧化物歧化酶(SOD),丙二醛(MDA),一氧化氮(NO),血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C),丙氨酸氨基转移酶(AST),天冬氨酸氨基转移酶(ALT),肌酸激酶(CK),肌酐(Cr)和尿素氮(BUN)水平,取胰腺苏木素-伊红(HE)染色观察组织结构。结果:与正常组比较,模型组的大鼠摄食量,饮水量,尿量,FBG,INS,MDA,NO,LDL-C,TC,TG,AST,ALT,BUN,Cr,CK水平均明显升高,CAT,SOD,HDL-C水平降低(P<0.05,P<0.01);与模型组比较,各给药组大鼠摄食量,饮水量,尿量,FBG,INS,MDA,NO,LDL-C,TC,TG,AST,ALT,BUN,Cr,CK水平均明显降低,CAT,SOD,HDLC水平升高(P<0.05,P<0.01);与正常组比较,模型组大鼠胰岛细胞数目减少,排列紊乱,颜色深,细胞间质和细胞间界限不清晰;与模型组比较,各给药组大鼠胰岛细胞数目增多,排列整齐,细胞形态恢复规则。结论:木瓜苦桑片有明显降血糖及血脂作用,可增强机体抗氧化损伤的能力,对肝肾功能具有明显改善作用,可维持胰岛正常结构和功能。Objective：To investigate the effects of Mugua Kusang tablets on blood glucose,lipid,function of liver,kidney and pancreas issues in type 2 diabetes rats induced by high sucrose/high fat diet combined wih low dose streptozotocin（STZ）.Method：Diabetes model was induced by feeding with high sucrose/high fat diet for 4 weeks and STZ at dose of 45 mg·kg-1injection in male SD rats,then the successfully modeled rats were divided randomly into the model group,metformin group（285 mg·kg-1）,and Mugua Kusang tablets high dose,medium dose and low dose groups（2 600,1 300,650 mg·kg-1）.Another 10 normal rats were assigned as the normal control group,n=10 in each group.Intragastric administration was given once a day for 4 weeks,and 24 h urine volume,water intake,and food intake were recorded for all rats.Then the levels of fasting blood glucose（FBG）,insulin（INS）,catalase（CAT）,superoxide dismutase（SOD）,methane dicarboxylic aldehyde（MDA）,nitric oxide（NO）,serum total cholesterol（TC）,triglyceride（TG）,low density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）,alanine aminotransferase（AST）,aspartate transaminase（ALT）,creatine kinase（CK）,creatinine（Cr） and urea nitrogen（BUN） were measured,and pancreatic structural change was observed by htoxylin-eosin（HE） staining.Result：As compared to the normal control group,the levels of food-intake,water-intake,urine volume,FBG,INS,MDA,NO,LDL-C,TC,TG,AST,ALT,BUN,CK and Cr were increased,while levels of CAT,SOD and HDL-C were decreased in the model group（P〈0.05,P〈0.01）.As compared to the model group,the levels of food-intake,water-intake,urine volume,FBG,INS,MDA,NO,LDL-C,TC,TG,AST,ALT,BUN,CK and Cr were decreased,while the levels of CAT,SOD and HDL-C were increased in all treatment groups（P〈0.05,P〈0.01）.As compared to the normal control group,islet cells count was decreased,with a disordered arrangement and darker color in model group,and the boundaries were indistinct betw

关 键 词：木瓜苦桑片 Ⅱ型糖尿病 血糖 胰腺 抗氧化 

分 类 号：R285.5[医药卫生—中药学]